Leydig-Cell Responsiveness to LH-hCG Stimulation: Mechanisms of hCG- and Steroid-Induced Refractoriness

  • F. Haour
  • J. M. Saez
Part of the Biochemical Endocrinology book series (BIOEND)


Since the discovery of polypeptide hormone receptors, sensitivity or refractoriness of a given cell to hormonal stimulation has been known to be related to the presence of the hormone receptor (Roth, 1973). The appearance of receptors and cell responsiveness has also been related to cell differentiation and maturation (Engel et al.,1973; Engel and Frowein, 1974). In recent years, it has been demonstrated that cell responsiveness may also vary according to preexposure of the target cells to the hormone (Tata, 1975; Raff, 1976; Kolata, 1977; Tell et al., 1978).


Leydig Cell cAMP Production Plasma Testosterone Luteinizing Hormone Receptor Testosterone Production 
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  1. Asling, C. W., Reinhart, W. O., and Li, C. H., 1951, Effect of adenocorticotropic hormone on body growth, visceral proportion and white blood cell counts of normal and hypophysectomized male rats, Endocrinology 48: 534–547.PubMedCrossRefGoogle Scholar
  2. Bex, F. J., and Bartke, A., 1977, Testicular LH binding in the hamster: Modification by photoperiod and prolactin, Endocrinology 100: 1223–1226.PubMedCrossRefGoogle Scholar
  3. Bockaert, J., Hunzicker-Dunn, M., and Birnbaumer, L., 1976, Hormone-stimulated desensitization of hormone dependent adenyl cyclase: Dual action of luteinizing hormone on pig Graafian follicle membranes, J. Biol. Chem. 251: 2563–2663.Google Scholar
  4. Brinkmann, A. O., Mulder, E., Lameri-Stahlhofen, G. J. M., Mechielsen, M. J., and Van der Molen, H. J., 1972, An oestradiol receptor in rat testis interstitial tissue, FEBS Lett. 26: 301–305.PubMedCrossRefGoogle Scholar
  5. Catt, K. J., and Dufau, M. L., 1973, Interactions of LH and hCG with testicular gonadotropin receptors, Adv. Exp. Med. Biol. 36: 379–418.PubMedCrossRefGoogle Scholar
  6. Chen, Y. D. I., and Payne A. H., 1977, Regulation of testicular LH receptors by homologous hormone: In vitro studies on receptor occupancy and receptor loss, Biochem. Biophys. Res. Commun. 74: 1589–1596.PubMedCrossRefGoogle Scholar
  7. Chen, Y. I., Shan, M. S., and Payne, A. H., 1977, Steroid and FSH action on LH receptors and LH sensitive testicular responsiveness during sexual maturation of the rat, Mo!. Cell. Endocrinol. 8: 291–300.CrossRefGoogle Scholar
  8. Conti, M., Hardwood, J., Hsueh, A., Dufau, M. L., and Catt, K. J., 1976, Gonadotropin induced loss of hormone receptors and desensitization of adenylate cyclase in the ovary, J. Biol. Chem. 251: 7729–7731.PubMedGoogle Scholar
  9. de Meyts, P., Roth, J., Neville, D. M., Gavin, J., and Lesniak, M. A., 1973, Insulin interactions with its receptors: Experimental evidence for negative cooperativity, Biochem. Biophys. Res. Commun. 55: 154–161.PubMedCrossRefGoogle Scholar
  10. Desjardins, C., and Ewing, L. L., 1971, Testicular metabolism in adrenalectomized and corticosterone treated rats, Proc. Soc. Exp. Biol. Med. 137: 578–581.Google Scholar
  11. Eik-Nes, K. B., 1970, Synthesis and secretion of androstenedione and testosterone, in: The Androgens of the Testis ( K. B. Eik-Nes, ed.), pp. 1–39, Marcel Dekker, New York.Google Scholar
  12. Engel, W., and Frowein, J., 1974, Development of hCG sensitivity in the testis of the immature rat, Cell 2: 75–80.PubMedCrossRefGoogle Scholar
  13. Engel, W., Frowein, J., and Weise, H. C., 1973, hCG receptors present in the gonadotropin insensitive Leydig cell of the immature rat, Nature (London) 240: 148–149.Google Scholar
  14. Evain, D., Morera, A. M., and Saez, J. M., 1976, Glucocorticoid receptors in interstitial cells of the rat testis, J. Steroid Biochem. 7: 1135–1139.PubMedCrossRefGoogle Scholar
  15. Forest, M. G., Cathiard, A. M., and Bertrand, J., 1973, Total and unbound testosterone levels in the newborn and in normal hypogonadal children: Use of a sensitive radio-immunoassay for testosterone, J. Clin. Endocrinol. Metab. 36: 1132–1142.PubMedCrossRefGoogle Scholar
  16. Gavin, J. R., III, Roth, J., Neville, D. M., de Meyts, P., and Buell, D. N., 1974, Insulin-dependent regulation of insulin receptor concentrations: A direct demonstration in cell culture, Proc. Natl. Acad. Sci. U.S.A. 71: 84–88.PubMedCrossRefGoogle Scholar
  17. Hall, P. F., 1970, Gonadotropic regulation of testicular function, in: The Androgens of the Testis ( K. B. Eik-Nes, ed.), pp. 73–116, Marcel Dekker, New York.Google Scholar
  18. Haour, F., and Lang, B., 1978, Role des récepteurs hormonaux dans la régulation du corps jaune, Pathol. Biol. (Paris) 26: 197–204.Google Scholar
  19. Haour, F., and Saez, J. M., 1977, Regulation by hCG of gonadotropin receptors in testicular Leydig cells: Evidence for a down regulation, Mol. Cell. Endocrinol. 7: 17–24.PubMedCrossRefGoogle Scholar
  20. Haour, F., and Saxena, B. B., 1974a, Characterization and solubilization of gonadotropin receptor of bovine corpus luteum, J. Biol. Chem. 249: 2185–2205.Google Scholar
  21. Haour, F., and Saxena, B. B., 1974b, Detection of gonadotropin in rabbit blastocyst prior to implantation, Science 185: 444.PubMedCrossRefGoogle Scholar
  22. Haour, F., Sanchez, P., Gallet, D., and Saez, J. M., 1977, Desensibilisation par la gonadotrophine chorionique humaine: Mise en évidence d’un contrôle négatif des récepteurs des gonadotrophines par l’hCG au niveau des cellules de Leydig du testicule, C. R. Acad. Sci. (Paris) 284: 1429–1432.Google Scholar
  23. Hinkle, P. M., and Tashjian, A. H. J., 1975, Thyroid-releasing hormone regulates the number of its own receptors in the GH3-strain of pituitary cells in culture, Biochemistry 14: 3845–3851.PubMedCrossRefGoogle Scholar
  24. Hsueh, A. J. W., DuPau, M. L., and Catt, K. J., 1976, Regulation of luteinizing hormone receptors in testicular interstitial cells by gonadotropins,Biochem. Biophys. Res. Commun. 72: 1145–1152.CrossRefGoogle Scholar
  25. Hsueh, R. J. W., Dufau, M. L., and Catt, K. J., 1977, Gonadotropin induced regulation of luteinizing hormone receptors and desensitization of testicular 3’5’-cyclic AMP and testosterone responses, Proc. Natl. Acad. Sci. U.S.A. 74: 592–595.PubMedCrossRefGoogle Scholar
  26. Jones, T. M., Fang, V. S., Landan, R. L., and Rosenfield, R., 1975, Direct inhibition of Leydig cell function by estradiol, Endocrinology 96:A-196.CrossRefGoogle Scholar
  27. Kimura, T., 1969, Effect of hypophysectomy and ACTH administration on the level of cholesterol side chain desmolase, Endocrinology 84: 492–499.CrossRefGoogle Scholar
  28. Kirschner, M. A., Wider, J. A., and Roos, G. T., 1970, Leydig cell function in men with gonadotrophin producing testicular tumors, J. Clin. Endocrinol. Metab. 30: 504–511.PubMedCrossRefGoogle Scholar
  29. Kirschner, M. A., Cohen, F. B., and Jespersen, D., 1974, Estrogen production and its origin in men with gonadotropin-producing neoplasms, J. Clin. Endocrinol. Metab. 39: 112–118.PubMedCrossRefGoogle Scholar
  30. Kolata, G. B., 1977, How are they regulated?, Science 196: 747–748.PubMedCrossRefGoogle Scholar
  31. Morera, A. M., and Saez, J. M., 1977, Désensibilisation des cellules surrénaliennes tumor-ales Y1 par l’ACTH et le DbcAMP, C. R. Acad. Sci. (Paris) 285: 1471–1475.Google Scholar
  32. Murota, S., Shikita, M., and Tamaoki, B. I., 1966, Androgen formation in the testicular tis-sue of patients with prostatic carcinoma, Biochim. Biophys. Acta 117: 241–246.CrossRefGoogle Scholar
  33. Odell, W. D., and Swerdloff, R. J., 1976, Etiologics of sexual maturation: A model system based on the sexually maturing rat, Recent Prog. Horm. Res. 32: 245–288.PubMedGoogle Scholar
  34. Oshima, M., Wakabayashi, K., and Tamaoki, B., 1967, The effect of synthetic estrogens upon the biosynthesis in vitro of androgen and luteinizing hormone in the rat, Biochim. Biophys. Acta 137: 356–366.PubMedCrossRefGoogle Scholar
  35. Posner, B. I., Kelly, P. A., and Friesen, H. G., 1975, Prolactin receptors in rat liver: Possible induction by prolactin, Science 188: 57–58.PubMedCrossRefGoogle Scholar
  36. Raff, M., 1976, Self regulation of membrane receptors, Nature (London) 259:265–266. Rappaport, B., and Adams, R. S., 1976, Induction of refractoriness to thyrotropin stimulation in cultured thyroid cells, J. Biol. Chem. 251: 6653–6661.Google Scholar
  37. Reiter, E. D., and Kulin, H. E., 1975, Plasma testosterone response to short-term human chorionic gonadotropin administration in men with follicle-stimulating hormone suppressed by exogenous estrogens, Fertil. Steril. 26: 340–345.PubMedGoogle Scholar
  38. Richards, J. S., and Midgley, A. R. V., 1976, Protein hormone action: A key to under- standing ovarian follicular and luteal cell development, Biol. Reprod. 14: 82–94.PubMedCrossRefGoogle Scholar
  39. Rommerts, F. F. G., Cooke, B. A., and Van der Molen, H. J., 1974, The role of cyclic AMP in the regulation of steroid biosynthesis in testis tissue, J. Steroid Biochem. 5: 279–285.PubMedCrossRefGoogle Scholar
  40. Roth, J., 1973, Peptide hormone binding to receptors: A review of direct studies in vitro, Metabolism 22: 1059–1073.PubMedCrossRefGoogle Scholar
  41. Saez, J M., Morera, A. M., Haour, F., and Evain, D., 1977, Effects of in vivo administration of dexamethasone ACTH and hCG on steroidogenesis and protein and DNA synthesis of testicular interstitial cells in prepubertal rats, Endocrinology 101: 1256–1263.PubMedCrossRefGoogle Scholar
  42. Saez, J. M., Haour, F., Loras, B., Sanchez, P., and Cathiard, A. M., 1978a, Estrogen induced Leydig cells refractoriness to gonadotropin stimulation, Acta Endocrinol. (in press).Google Scholar
  43. Saez, J. M., Haour, F., Tell, G. P. E., Gallet, D., Sanchez, P., 1978b, hCG-induced Leydig cells refractoriness to gonadotropin stimulation, Mol. Pharmacol. 14 (Sept.) (in press).Google Scholar
  44. Sharpe, R. M., 1976, hCG-induced decrease in availability of rat testis receptors, Nature (London) 264: 644–646.PubMedCrossRefGoogle Scholar
  45. Sharpe, R. M., 1977a, Relationship between testosterone fluid constant and luteinizing hor- mone receptor in the rat testis, Biochem. Biophys. Res. Commun. 75: 711–717.CrossRefGoogle Scholar
  46. Sharpe, R. M., 1976, Gonadotrophin induced reduction in the steroidogenic responsi- veness of the immature rat testis, Biochem. Biophys. Res. Commun. 76: 957–962.CrossRefGoogle Scholar
  47. Sholiton, L. J., Srivastava, L., and Taylor, B. B., 1975, The in vitro andin vivo effects of diethylstilbestrol on testicular synthesis of testosterone, Steroids 26: 797–806.PubMedCrossRefGoogle Scholar
  48. Slaunwhite, W. R., Sandberg, A. A., Jackson, J. E., and Staubitz, W. J., 1962, Effects of estrogens and hCG on androgen synthesis by human testis, J. Clin. Endocrinol. Metab. 22: 992.PubMedCrossRefGoogle Scholar
  49. Smals, A. G. H., Kloppenbord, P. W. C., and Benraad, Th. J., 1977, Plasma testosterone profiles in Cushing’s syndrome, J. Clin. Endocrinol. Metab. 45: 240–245.PubMedCrossRefGoogle Scholar
  50. Sugiyama, H., Popot, J. L., and Changeux, J. P., 1976, Studies on the electrogenic action of acetylcholine with Torpedo marmorata electric organ. III. Pharmacological desensitization in vitro of the receptor-rich membrane fragments by cholinergic agonists, J. Mol. Biol. 106: 485–496.PubMedCrossRefGoogle Scholar
  51. Tata, J. R., 1975, Hormonal regulation of hormone receptors, Nature (London) 257: 740–741.CrossRefGoogle Scholar
  52. Tell, G. P. E., Haour, F., and Saez, J. M., 1978, Hormonal regulation of membrane receptors and cell responsiveness: A review, Metabolism 14(4) (in press).Google Scholar
  53. Verjans, H. L., and Eik-Nes, K. B., 1976, Hypothalamic—pituitary testicular system and adrenocortical function, Acta Endocrinol. (Copenhagen) 81: 198–207.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • F. Haour
    • 1
  • J. M. Saez
    • 1
  1. 1.Unité de Recherches sur le Contrôle Hormonal des Activités CellulairesINSERM-U.162. Hôital DebrousseLyon, Cedex 1France

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