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Glycoprotein Hormones and Their Subunits—Immunological and Biological Characterization

  • Judith L. Vaitukaitis
Part of the Biochemical Endocrinology book series (BIOEND)

Abstract

With the advent of more sophisticated purification and isolation techniques for the glycoprotein hormones and their subunits, reagents have been generated to foster a better understanding of the immunological and physiological control of glycoprotein hormone synthesis and secretion. The human glycoproteins—luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG)—are all composed of a protein core with branched carbohydrate side chains usually terminating with sialylic acid.

Keywords

Luteinizing Hormone Human Chorionic Gonadotropin Elution Profile Glycoprotein Hormone Gestational Trophoblastic Disease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aloj, S. M., Edelhoch, H., Ingham, K. C., Morgan, F. J., Canfield, R. E., and Ross, G. T., 1973, The rates of dissociation and reassociation of the subunits of human chorionic gonadotropin, Arch. Biochem. Biophys. 159: 497.PubMedCrossRefGoogle Scholar
  2. Arnheim, N., Sobel, J., and Canfield, R., 1971, Immunochemical resemblance between human leukemia and hen egg-white lysozyme and their reduced carboxymethyl derivatives, J. Mol. Biol. 61: 237.PubMedCrossRefGoogle Scholar
  3. Ashitaka, Y., Nishimura, R., Endoh, Y., and Tojo, S., 1974a, Subunits of human chorionic gonadotropin and their radioimmunoassays, Endocrinol. Jpn. 21: 429.PubMedCrossRefGoogle Scholar
  4. Ashitaka, Y., Nishimura, R., Ohashi, M., Futamura, K., and Tojo, S., 1974b, Serum and chorionic tissue concentrations of human chorionic gonadotropin and its subunits during pregnancy, Endocrinol. Jpn. 21: 547.PubMedCrossRefGoogle Scholar
  5. Ashitaka, Y., Nishimura, R., Futamura, K., and Tojo, S., 1977, Alpha subunit of human chorionic gonadotropin in fluid of molar vesicles, Endocrinol. Jpn. 24: 115.PubMedCrossRefGoogle Scholar
  6. Bellisario, R., Carlsen, R. B., and Bahl, O. P., 1973, Human chorionic gonadotropin: Linear amino acid sequence of the a subunit, J. Biol. Chem. 248: 6796.PubMedGoogle Scholar
  7. Blake, C. C. F., and Swan, I. D. A., 1971, X-ray analysis of structure of human lysozymes at 6 Å resolution, Nature (London) New Biol. 232: 72.Google Scholar
  8. Braunstein, G. D., Vaitukaitis, J. L., and Ross, G. T., 1972, The in vitro behavior of human chorionic gonadotropin after dissociation into subunits, Endocrinology 91: 1030.PubMedCrossRefGoogle Scholar
  9. Braunstein, G. D., Grodin, J. M., Vaitukaitis, J., and Ross, G. T., 1973a, Secretory rates of human chorionic gonadotropin by normal trophoblast, Am. J. Obstet. Gynecol. 115: 447.PubMedGoogle Scholar
  10. Braunstein, G. D., Vaitukaitis, J. L., Carbone, P. P., and Ross, G. T., 1973b, Ectopic production of human chorionic gonadotropin by neoplasms, Ann. Intern Med. 78: 39.PubMedGoogle Scholar
  11. Braunstein, G. D., McIntire, K. R., and Waldemann, T. A., 1973c, Discordance of human chorionic gonadotropin and alpha-fetoprotein in testicular teratocarcinomas, Cancer 31: 1065.PubMedCrossRefGoogle Scholar
  12. Braunstein, G. D., Rasor, J., and Wade, M. E., 1975, Presence in normal human testis of a chorionic-gonadotropin-like substance distinct from human luteinizing hormone, New Engl. J. Med. 293: 1339.PubMedCrossRefGoogle Scholar
  13. Canfield, R. E., Kammerman, S., Sobel, J. H., and Morgan, F., 1971, Primary structure of lysozymes from man and goose, Nature (London) New Biol. 232: 16.Google Scholar
  14. Carlsen, R. B., Bahl, O. P., and Swaminathan, N., 1973, Human chorionic gonadotropin: Linear amino acid sequence of the β subunit, J. Biol. Chem. 248: 6810.PubMedGoogle Scholar
  15. Catt, K. J., Dufau, J. L., and Tsuruhara, T., 1973, Absence of intrinsic biological activity in LH and CG subunits, J. Clin. Endocrinol. Metab. 36: 73.PubMedCrossRefGoogle Scholar
  16. Catt, K. J., Dufau, M. L., and Vaitukaitis, J. L., 1975, Appearance of hCG in pregnancy plasma following the initiation of implantation of the blastocyst, J. Clin. Endocrinol. Metab. 40: 537.PubMedCrossRefGoogle Scholar
  17. Chen, H.-C., Hodgen, G. T., Matsuma, S., Lin, L. J., Gross, E., Reichert, L. E., Jr., Birken, S., Canfield, R. E., and Ross, G. T., 1976, Evidence for a gonadotropin from non-pregnant subjects that has physical, immunological and biological similarities to human chorionic gonadotropin, Proc. Natl. Acad. Sci. U.S.A. 73: 2885.PubMedCrossRefGoogle Scholar
  18. Closset, J., Hennen, G., and Lequin, R. M., 1975, Human luteinizing hormone: The amino acid sequence of the β subunit, FEBS Lett. 29: 97.CrossRefGoogle Scholar
  19. Cohen, H., and Stramp, L. A., 1976, Bacterial synthesis of substance similar to human chorionic gonadotropin, Proc. Soc. Exp. Biol. Med. 152: 408.PubMedGoogle Scholar
  20. Dubois, P. M., and Dubois, M. P., 1974, Mise en evidence pour immunofluorescence de l’activité gonadotrope LH dans l’ante-hypophyse foetale humaine, in: Endocrinologie Sexuelle de la Periode Perinatale, p. 37, Editions Paris.Google Scholar
  21. Edmonds, M., Molitch, M., Pierce, J. G., and Odell, W. D., 1975, Secretion of a subunits of luteinizing hormone (LH) by the anterior pituitary, J. Clin. Endocrinol. Metab. 41: 551.PubMedCrossRefGoogle Scholar
  22. Franchimont, P., and Pasteels, J. L., 1972, Secretion independante des hormones gonadotropes et de leurs sous-unités, C. R. Acad. Sci. 275: 1799.Google Scholar
  23. Franchimont, P., and Reuter, A., 1972, Evidence of a and β subunits of hCG in serum and urine of pregnant women, in: Protein and Polypeptide Hormones (M. Margoulies and F. Greenwood, eds.), p. 381, Excerpta Medica, Amsterdam.Google Scholar
  24. Fusco, F. D., and Rosen, S. W., 1966, Gonadotropin-producing anaplastic large-cell carcinoma of the lung, N. Engl. J. Med. 275: 507.PubMedCrossRefGoogle Scholar
  25. Ghose, N. K., and Cox, R. P., 1976, Production of human chorionic gonadotropin in HeLa cell cultures, Nature (London) 259: 416.CrossRefGoogle Scholar
  26. Hagen, C., and McNeilly, A. S., 1975, The gonadotropic hormones and their subunits in human maternal and fetal circulation at delivery, Am. J. Obstet. Gynecol. 121: 926.PubMedGoogle Scholar
  27. Hobson, B., and Wide, L., 1968, Human chorionic gonadotropin excretion in men and women with invasive trophoblast assayed by an immunological and a biological method, Acta Endocrinol. (Copenhagen) 58: 473.Google Scholar
  28. Hung, W., Blizzard, R. M., Migeon, C. J., Camacho, A. M., and Nyhan, W. L., 1963, Precocious puberty in a boy with hepatoma and circulating gonadotròpin, Pediatrics 63: 895.CrossRefGoogle Scholar
  29. Kaplan, S. L., Grumbach, M. M., and Aubert, M. L., 1976, α and ß glycoprotein hormone subunits (hLH, hFSH, hCG) in the serum and pituitary of the human fetus, J. Clin. Endocrinol. Metab. 42: 995.Google Scholar
  30. Kourides, I. A., Weintraub, B. D., Ridgway, E. C., and Maloof, F., 1973, Increase in the ß subunit of human TSH in hypothyroid serum after thyrotropin releasing hormone, J. Clin. Endocrinol. Metab. 37: 836.PubMedCrossRefGoogle Scholar
  31. Kourides, I. A., Weintraub, B. D., Ridgway, E. C., and Maloof, F., 1975, Pituitary secretion of free alpha and beta subunit of human thyrotropin in patients with thyroid disorders, J. Clin. Endocrinol. Metab. 40: 872.PubMedCrossRefGoogle Scholar
  32. Lerario, A. C., Pierce, J. G., and Vaitukaitis, J. L., 1978, Effect of conformation of hCG on generation of hCG-specific antibody, Endocr. Res. Commun. (in press).Google Scholar
  33. Louvet, J. P., Ross, G. T., Birken, S., and Canfield, R. E., 1974, Absence of neutralizing effect of antisera to the unique structural region of human chorionic gonadotropin, J. Clin. Endocrinol. Metab. 39: 1155.PubMedCrossRefGoogle Scholar
  34. MacNab, G. H., Moncrieff, S. A., and Budian, M., 1952, Primary malignant hepatic tumors in childhood, in: British Empire Cancer Campaign 30th Annual Report, p. 168, Sunfield and Day, Eastbourne, Sussex.Google Scholar
  35. Mishell, D. R., Nakamura, R. M., Barberia, J. M., and Thorneycroft, I. H., 1974, Initial detection of human chorionic gonadotropin in serum in normal human gestation, Am. J. Obstet. Gynecol. 118: 990.PubMedGoogle Scholar
  36. Morgan, F. J., Birken, S., and Canfield, R. E., 1973a, Human chorionic gonadotropin: A proposal for the amino acid sequence, Mol. Cell. Biochem. 2: 97.PubMedCrossRefGoogle Scholar
  37. Morgan, F. J., Canfield, R. E., Vaitukaitis, J. L., and Ross, G. T., 1973b, Preparation and characterization of the subunits of hCG, in: Peptide Hormones ( S. A. Berson and R. S. Yalow, eds.), pp. 733–742, North-Holland, Amsterdam.Google Scholar
  38. Morgan, F. J., Birken, S., and Canfield, R. E., 1975, The amino acid sequence of human chorionic gonadotropin, J. Biol. Chem. 250: 5247.PubMedGoogle Scholar
  39. Odell, W. A., Abraham, G., Rand, H. R., Swerdloff, R. S., and Fisher, D., 1969, Influence of immunization procedures on the titer, affinity and specificity of antisera to glycopolypeptides, Acta Endocrinol. (Copenhagen) Suppl. 142: 54.Google Scholar
  40. Pierce, J. G., Liao, T. H., Howard, S. M., Shome, B., and Cornell, J. S., 1971, Studies on the structure of thyrotropins: Its relationship to luteinizing hormone, Recent Prog. Horm. Res. 27: 165.PubMedGoogle Scholar
  41. Prentice, L. G., and Ryan, R. J., 1975, LH and its subunits in human pituitary, serum and urine, J. Clin. Endocrinol. Metab. 40: 303.PubMedCrossRefGoogle Scholar
  42. Reichert, L. E., Jr., Rasco, M. A., Ward, D. N., Niswender, G. D., and Midgley, A. R., Jr., 1969, Isolation and properties of subunits of bovine pituitary luteinizing hormone, J. Biol. Chem. 244: 5110.PubMedGoogle Scholar
  43. Reichert, L. E., Jr., Midgley, A. R., Jr., Niswender, G. D., and Ward, D. N., 1970, Formation of a hybrid molecule from subunits of human and bovine luteinizing hormone, Endocrinology 87: 534.PubMedCrossRefGoogle Scholar
  44. Reichert, N. D., and Ryan, R. J., 1977, Specific gonadotropin binding to Pseudomonas maltophila, Proc. Natl. Acad. Sci. U.S.A. 74: 878.CrossRefGoogle Scholar
  45. Rose, L. I., Williams, G. H., Jagger, P., and Lauler, D. P., 1968, Feminizing tumor of the adrenal gland with positive “chorionic” like “gonadotropin” test, J. Clin. Endocrinol. Metab. 28: 903.PubMedCrossRefGoogle Scholar
  46. Rosen, S. W., and Weintraub, B. D., 1974, Ectopic production of the isolated alpha subunit of the glycoprotein hormones: A quantitative marker in certain cases of cancer, N. Engl. J. Med. 290: 1441.PubMedCrossRefGoogle Scholar
  47. Rosen, S. W., Weintraub, B. D., Vaitukaitis, J. L., Sussman, H. H., Hershman, J. M., and Muggia, F. M., 1975, Placental proteins and their subunits as tumor markers, Ann. Intern Med. 82: 71.Google Scholar
  48. Schlaff, S., Rosen, S. W., and Roth J., 1968, Antibody to human follicle stimulating hormone: Cross reactivity with three other hormones, J. Clin. Invest. 47: 1722.PubMedCrossRefGoogle Scholar
  49. Shome, B., and Parlow, A. F., 1974a, Human follicle stimulating hormone (hFSH): First proposal for the amino acid sequence of the a subunit (hFSHa) and first demonstration of its identity with the α subunit of human luteinizing hormone (hLH-α), J. Clin. Endocrinol. Metab. 39: 191.CrossRefGoogle Scholar
  50. Shome, B., and Parlow, A. F., 1974b, Human follicle stimulating hormone: First proposal for the amino acid sequence of the hormone-specific β subunit (hFSH-ß), J. Endocrinol. Metab. 39: 203.CrossRefGoogle Scholar
  51. Vaitukaitis, J. L., 1973, Immunologic and physical characterization of human chorionic gonadotropin (hCG) secreted by tumors, J. Clin. Endocrinol. Metab. 37: 505.PubMedCrossRefGoogle Scholar
  52. Vaitukaitis, J. L., 1974, Changing placental concentrations of hCG and its subunits during gestation, J. Clin. Endocrinol. Metab. 38: 755.PubMedCrossRefGoogle Scholar
  53. Vaitukaitis, J. L., 1975, When is a tumor marker not a tumor marker?, N. Engl. J. Med. 293: 1370.PubMedCrossRefGoogle Scholar
  54. Vaitukaitis, J. L., and Ebersole, E. R., 1976, Evidence for altered synthesis of human chorionic gonadotropin in gestational trophoblastic tumors, J. Clin. Endocrinol. Metab. 42: 1048.PubMedCrossRefGoogle Scholar
  55. Vaitukaitis, J. L., and Ross, G. T., 1972, Antigenic similarities among the human glycoprotein hormones and their subunits, in: Gonadotropins ( B. Saxena, H. Gandy, and C. Beling, eds.), pp. 435–477, Wiley, New York.Google Scholar
  56. Vaitukaitis, J. L., Robbins, J. B., Nieschlag, E., and Ross, G. T., 1971, A method for producing specific antisera with small doses of immunogen, J. Clin. Endocrinol. Metab. 33: 988.PubMedCrossRefGoogle Scholar
  57. Vaitukaitis, J. L., Braunstein, G. D., and Ross, G. T., 1972a, A radioimmunoassay which specifically measures human chorionic gonadotropin in the presence of human luteinizing hormone, Am. J. Obstet. Gynecol. 113: 751.PubMedGoogle Scholar
  58. Vaitukaitis, J. L., Ross, G. T., Reichert, L. E., Jr., and Ward, D. N., 1972b, Immunologic basis for within and between species cross-reactivity of luteinizing hormone, Endocrinology 91: 1337.PubMedCrossRefGoogle Scholar
  59. Vaitukaitis, J. L., Ross, G. T., and Reichert, L. E., Jr., 1973, Immunologic and biologic behavior of hCG and bovine LH subunit hybrids, Endocrinology 92: 411.CrossRefGoogle Scholar
  60. Vaitukaitis, J. L., Ross, G. T., Braunstein, G. D., and Rayford, P. L., 1976, Gonadotropins and their subunits: Basic and clinical studies, Recent Frog. Horm. Res. 32: 289.Google Scholar
  61. Weintraub, B. D., and Rosen, S. W., 1973, Ectopic production of the isolated beta subunit of human chorionic gonadotropin, J. Clin. Invest. 52: 3135.PubMedCrossRefGoogle Scholar
  62. Wide, L., Roos, P., and Gemzel, C. A., 1961, Immunological determination of human pituitary luteinizing hormone (LH), Acta Endocrinol. (Copenhagen) 37: 445.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Judith L. Vaitukaitis
    • 1
    • 2
  1. 1.Thorndike Memorial Laboratory, Section of Endocrinology and MetabolismBoston City HospitalBostonUSA
  2. 2.Department of MedicineBoston University School of MedicineBostonUSA

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