Regulation of Gene Expression in the Nucleus by Gonadotropins

  • Kenneth W. McKerns
Part of the Biochemical Endocrinology book series (BIOEND)


For the past 15 years or so, I have been providing evidence that polypeptide hormones stimulate hormone-specific species of glucose-6-phosphate dehydrogenase (Glc-6-PD) in their target tissues. This allosteric regulation of the rate-limiting enzyme of the pentose phosphate cycle provides reducing equivalents for biosynthetic purposes. In addition, it provides ribose sugars such as 5-phospho-d-ribosyl pyrophosphate (5-PRPP) that are rate-limiting to the synthesis de novo of purine and pyrimidine nucleotides. These nucleotides are required in the synthesis of RNA and DNA and in electron- and energy-carrying compounds, such as NADPH and ATP.


Corpus Luteum Adrenal Cortex Pyrimidine Nucleotide Purine Biosynthesis Nuclear Gene Expression 

Abbreviations used in this chapter


Glucose-6-phosphate dehydrogenase




human choriogonadotropin




messenger RNA




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  1. Criss, E. E., and Mckems,K. W., 1968, Activation of cow adrenal glucose-6-phosphate dehydrogenase by adrenal corticotropin, Biochemistry 7: 2364–2368.PubMedCrossRefGoogle Scholar
  2. Criss, W. E., and McKerns, K. W., 1969, Studies on ACTH-induced changes at the NADP`-binding site of bovine adrenal glucose-6-phosphate dehydrogenase, Arch. Biochem. 135:118.PubMedCrossRefGoogle Scholar
  3. McKems, K. W., 1964, Mechanism of action of ACTH through activation of glucose-6phosphate dehydrogenase, Biochim. Biophys. Acta 90: 357–371.CrossRefGoogle Scholar
  4. McKerns, K. W., 1967, Genetic, biochemical and hormonal mechanisms in the regulation of uterine metabolism in: Cellular Biology of the Uterus (R. M. Wynn, ed.), pp. 71–113, Appleton-Century-Crofts, New York.Google Scholar
  5. McKerns, K. W., 1968, Mechanism of ACTH regulation of adrenal cortex, in: Functions of the Adrenal Cortex, pp. 479–533, Appleton-Century-Crofts, New York.Google Scholar
  6. McKerns, K. W., 1969a, Steroid Hormones and Metabolism, Appleton-Century-Crofts, New York.Google Scholar
  7. McKerns, K. W., 1969b, Studies on the regulation of ovarian function by gonadotropin, in: The Gonads ( K. W. McKerns, ed.), Appleton-Century-Crofts, New York.Google Scholar
  8. McKerns, K. W., 1973a, ACTH stimulation of purine nucleotide biosynthesis in the adrenalcortex, Arch. Biochem. Biophys. 154: 341.PubMedCrossRefGoogle Scholar
  9. McKerns, K. W., I973b, Gonadotropin regulation of nucleotide biosynthesis in corpus luteum, Biochemistry 12: 5206–5211.Google Scholar
  10. McKerns, K. W., 1976, Genetic, biochemical and hormonal mechanisms in the regulation of the uterus, in: Cellular Biology of the Uterus (R. M. Wynn, ed.), pp. 101–138, Plenum Press, New York.Google Scholar
  11. McKerns, K. W., and Ryschkewitsch, W., 1974, Interaction of luteinizing hormone and its subunits in corpus luteum supernatant, Endocrinology 95: 847–853.PubMedCrossRefGoogle Scholar
  12. McKerns, K. W., and Ryschkewitsch, W., 1976, Regulation of RNA synthesis in corporaGoogle Scholar
  13. lutea by luteinizing hormone and derivatives, Biochim. Biophys. Acta 454:51–54. McKerns, K. W., and Ryschkewitsch, W., 1977, Lutropin stimulation of RNA synthesis in corpus luteum chromatin, Biochim. Biophys. Acta 478:68–74.Google Scholar
  14. Péron, F. G., and Caldwell, B. V., 1968, Further studies on corticosteroidogenesis. V. 1lß-Hydroxylation of DOC by mitochondria incubated with malate supernatant fraction and supernatant fraction + pyruvate + CO2, Biochim. Biophys. Acta 164: 396.PubMedCrossRefGoogle Scholar
  15. Schwyzer, R., and Schiller, P. W., 1969, The role of adenyl cyclase and cyclic 3’,5’-AMP in biological systems, Fogarty Int. Center Proc., No. 4 (Nov. 17–19), pp. 105–111, Bethesda, Maryland.Google Scholar
  16. Simpson, E. R., Cammer, W., and Estabrook, R. W., 1968, The role of malic enzyme in bovine adrenal cortex mitochondria, Biochem. Biophys. Res. Commun. 31:113.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Kenneth W. McKerns
    • 1
  1. 1.Department of Obstetrics and GynecologyUniversity of Florida College of MedicineGainesvilleUSA

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