Effects of FSH and LH on RNA Synthesis in the Testis: Role of Ornithine Decarboxylase

  • Claude A. Villee
  • Janet M. Loring
Part of the Biochemical Endocrinology book series (BIOEND)


It has long been known that testicular size decreases following hypophysectomy and can be restored toward normal by the injection of pituitary extracts. The sequence of events involved in the response of the gonads to gonadotropins has been of interest to many investigators in recent years. Gonadotropic hormones increase the synthesis of RNA and protein in the testis of the rat (Means and Hall, 1967, 1968, 1969; Goswami, et al., 1968; Villee, D., and Goswami, 1973) and increase the production of cyclic AMP (cAMP) (Murad et al., 1969; Hollinger, 1970; Kuehl et al., 1970; Dorrington et al., 1972; Braun and Sepsenwol, 1974). The increased synthesis of RNA and protein in response to the hormone was inhibited by pretreatment of the animals with actinomycin D (Means and Hall, 1969; Goswami et al., 1968; Reddy and Villee, 1975a). Messenger RNA (mRNA) from animal origins has been shown to contain a polyadenylate sequence at the 3′ terminal end (Lim and Canellakis, 1970; Darnell eet al., 1971; Lee et al., 1971; Edmonds et al., 1971). The poly(A)-rich RNA can be conveniently isolated by affinity chromatography using oligo(dT) columns (Aviv and Leder, 1972).


Luteinizing Hormone Interstitial Cell Seminiferous Tubule Ornithine Decarboxylase Dibutyryl cAMP 
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Abbreviations used in this chapter


cyclic AMP

C subunit

catalytic subunit of protein kinase


human chorionic gonadotropin


follicle-stimulating hormone


luteinizing hormone


1-methyl-3-isobutyl xanthine


messenger RNA


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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Claude A. Villee
    • 1
  • Janet M. Loring
    • 1
  1. 1.Department of Biological Chemistry and Laboratory of Human Reproduction and Reproductive BiologyHarvard Medical SchoolBostonUSA

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