Drug Effects on Lutropin Action

  • M. R. Sairam
Part of the Biochemical Endocrinology book series (BIOEND)


Stimulation of gonadal steroidogenesis resulting in the secretion of sex steroids is one of the well-known actions of pituitary lutropin. The mechanism of action of lutropin has been widely investigated by both in vivo and in vitro studies in the male and female. In recent years, improvements in the technique of obtaining dispersed cells from tissues as well as isolating specific cell types and procedures for culturing have greatly facilitated the studies on mechanism of action of hormones (O’Malley and Hardman, 1975). Hormone-responsive Leydig cells from rat testis can be prepared easily by appropriate digestion with collagenase followed by gentle dispersion (Moyle and Ramachandran, 1973; Dufau and Catt, 1975). We have utilized these collagenase-dispersed Leydig cells in our current investigation on the effect of a variety of drugs on lutropin action.


Leydig Cell Antimalarial Drug Quinine Sulfate Dibutyryl cAMP Pregnant Mare Serum Gonadotropin 
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Abbreviations used in this chapter


bovine serum albumin


cyclic AMP


human chorionic gonadotropin


luteinizing hormone (of ovine origin unless otherwise indicated)


1-methyl-3-isobutyl xanthine






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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • M. R. Sairam
    • 1
    • 2
  1. 1.Reproduction Research LaboratoryClinical Research Institute of MontrealMontrealCanada
  2. 2.University of MontrealMontrealCanada

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