Drug Effects on Lutropin Action
Stimulation of gonadal steroidogenesis resulting in the secretion of sex steroids is one of the well-known actions of pituitary lutropin. The mechanism of action of lutropin has been widely investigated by both in vivo and in vitro studies in the male and female. In recent years, improvements in the technique of obtaining dispersed cells from tissues as well as isolating specific cell types and procedures for culturing have greatly facilitated the studies on mechanism of action of hormones (O’Malley and Hardman, 1975). Hormone-responsive Leydig cells from rat testis can be prepared easily by appropriate digestion with collagenase followed by gentle dispersion (Moyle and Ramachandran, 1973; Dufau and Catt, 1975). We have utilized these collagenase-dispersed Leydig cells in our current investigation on the effect of a variety of drugs on lutropin action.
KeywordsLeydig Cell Antimalarial Drug Quinine Sulfate Dibutyryl cAMP Pregnant Mare Serum Gonadotropin
Abbreviations used in this chapter
bovine serum albumin
human chorionic gonadotropin
luteinizing hormone (of ovine origin unless otherwise indicated)
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