The Biosynthesis of Chorionic Gonadotropin and Placental Lactogen in First- and Third-Trimester Human Placenta

  • Irving Boime
  • Thomas Landefeld
  • Susan McQueen
  • Diana McWilliams
Part of the Biochemical Endocrinology book series (BIOEND)


One of the important functions of the human placenta is its role in the formation of peptide hormones during pregnancy. The major protein hormones elaborated by the trophoblast are human chorionic gonadotropin (hCG) and human placental lactogen (hPL). The concentrations of these hormones in maternal serum during gestation are quite different. Whereas hCG peaks in the first trimester, hPL reaches maximal levels near term. Since it seems, then, that these two hormones differ in the way their synthesis is correlated with the course of pregnancy, it is apparent that the factors controlling their synthesis are also quite different. Thus, the placenta represents a convenient and unique tissue for studying control of human hormonal genes under the influence of a variety of physiological stimuli.


Human Chorionic Gonadotropin Term Placenta Placental Lactogen Human Placental Lactogen Ectopic Tumor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used in this chapter


complementary DNA


human chorionic gonadotropin


human placental lactogen (also human chorionic somatomammotropin)


messenger RNA

S-30, S-100

30,000g and 100,000g supernatants


sodium dodecyl sulfate


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  1. Ashitaka, Y., Nishimura, R., Fatamura, K., Ohashi, M. and Togo, S., 1974, Serum and chorionic tissue concentrations of human chorionic gonadotropin and its subunits during pregnancy, Endocrinol. Jpn. 21: 547–550.PubMedCrossRefGoogle Scholar
  2. Bahl, 0., 1977, Human chorionic gonadotropin, its receptor and mechanism of action, Fed. Proc. Fed. Am. Soc. Exp. Biol. 36: 2119–2127.Google Scholar
  3. Boime, I., and Boguslawski, S., 1974, The synthesis of human placental lactogen by ribo- somes derived from human placenta, Proc. Natl. Acad. Sci. U.S.A. 71: 1322–1325.PubMedCrossRefGoogle Scholar
  4. Boime, I., McWilliams, D., Szczesna, E., and Camel, M., 1976, Synthesis of human placental lactogen mRNA as a function of gestation, J. Biol. Chem. 251: 820–825.PubMedGoogle Scholar
  5. Boime, I., Szczesna, E., and Smith, D., 1977, Membrane-dependent conversion of the human placental lactogen precursor to its native form in ascites cell-free extracts, Eur. J. Biochem. 73: 515–520.PubMedCrossRefGoogle Scholar
  6. Chatterjee, M., and Munro, H., 1977, Changing ratio of human chorionic gonadotropin sub-units synthesized by early and full-term placental polyribosomes, Biochem. Biophys. Res. Commun. 77: 426–433.PubMedCrossRefGoogle Scholar
  7. Chatterjee, M., Baliga, B., and Munro, H., 1976, Synthesis of human placental lactogen and human chorionic gonadotropin by polyribosomes and messenger RNAs from early and full term placentas, J. Biol. Chem. 251: 2945.PubMedGoogle Scholar
  8. Crosignani, P. G., 1974, Correlation of human chorionic gonadotropin and somatomammotropin with fetal nutrition, in: Lactogenic Hormones, Fetal Nutrition and Lactation ( J. Josimovich, M. Reynolds, and E. Cobo, eds.), pp. 203–217, Wiley Press, New York.Google Scholar
  9. Dawood, M., Saxena, B., and Landesman, R., 1977, Human chorionic gonadotropin and its subunits in hydatiform mole and choriocarcinoma, Obstet. Gynecol. 50: 172–181.PubMedGoogle Scholar
  10. Dreskin, R., Spicer, S., and Greene, W., 1970, Ultrastructural localization of chorionic gonadotropin in human term placenta, J. Histochem. Cytol. 18: 862–872.CrossRefGoogle Scholar
  11. Kaplan, S., and Grumbach, 1974, Effects of primate chorionic somatomammotropin on maternal and fetal metabolism, in: Lactogenic Hormones, Fetal Nutrition and Lactation ( Kaplan, S., and Grumbach, eds.), pp. 183–191, Wiley Press.Google Scholar
  12. Landefeld, T., McWilliams, D., and Boime, I., 1976a, The isolation of mRNA encoding the α subunit of human chorionic gonadotropin, Biochem. Biophys. Res. Commun. 72: 381–390.PubMedCrossRefGoogle Scholar
  13. Landefeld, T. D., Boguslawski, S., Corash. L., and Boime, I., 1976b, The cell-free syn-thesis of the αsubunit of human choionic gonadotropia, Endocrinology 98:1220–1227.Google Scholar
  14. Laskey, R., and Mills, A., 1975, Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography, Eur. J. Biochem. 56: 335–341.Google Scholar
  15. McWilliams, D., Callahan, R., and Boime, I., 1977, Human placental lactogen mRNA and its structural genes during pregnancy: Quantitation with a complementary DNA, Proc. Natl. Acad. Sci. U.S.A. 74: 1024–1028.PubMedCrossRefGoogle Scholar
  16. Morgan F., Birken, S., and Canfield, R., 1975, The amino acid sequence of human chorionic gonadotropin, J. Biol. Chem. 250: 5247–5258.PubMedGoogle Scholar
  17. Prentice, L., and Ryan, R. J., 1975, LH and its subunits in human pituitary, serum and urine, J. Clin. Endocrinol. Metab. 40: 303–312.PubMedCrossRefGoogle Scholar
  18. Thiede, H., and Choate, J. W., 1%3, Chorionic gonadotropin localization in the human placenta by immunofluorescent staining, Obstet. Gynecol. 22: 433–443.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Irving Boime
    • 1
  • Thomas Landefeld
    • 1
  • Susan McQueen
    • 1
  • Diana McWilliams
    • 1
  1. 1.Departments of Obstetrics and GynecologyWashington University School of MedicineSt. LouisUSA

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