Abstract
During the last years dramatic advances have been made with regard to the possibility to analyse the nervous system, not at least the hypothalamus, with histochemical methods. This is in part due to the fact that immunohistochemical techniques have been employed in the analysis and identification of specific neuron systems. The immunohistochemical approach is characterized by its unique general applicability, since principally any substance can be traced against which antibodies can be raised. Immunohisto-chemistry was originally introduced more than thirty years ago by Coons and collaborators (see 1). It was successfully applied to transmitter related problems by Geffen et al. (2) who identified catecholamine neurons with antibodies to dopamine-β-hydroxylase, the enzyme converting dopamine to noradrenaline. Now the phase has been reached where the full capacity of this technique can be exploited in studies on the nervous system. This is a consequence of the fact that only now have a larger number of enzymes and peptides been purified and structurally characterized in a way that they can be utilized as proper antigens for raising antisera.
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Hökfelt, T. et al. (1979). Neurotransmitters and Neuropeptides: Distribution Patterns and Cellular Localization as Revealed by Immunocytochemistry. In: Fuxe, K., Hökfelt, T., Luft, R. (eds) Central Regulation of the Endocrine System. Published Nobel Symposia, vol 42. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3396-8_4
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