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Antibodies to Allozymes as Potential Cell Markers for Chimeric Mouse Studies

  • John Gearhart
  • Mary Lou Oster-Granite
Part of the Basic Life Sciences book series (BLSC, volume 12)

Abstract

The limiting factor in the use of mouse chimeras for the revelation of detailed fate maps during embryogenesis is the lack of suitable cell marker systems. This limitation is evident when the list of available chimeric or mosaic markers is examined and compared to the criteria for an ideal marker system. An ideal cell marker should (1) be expressed in all descendants of a cell; (2) be present in all cells of a tissue or organism; (3) be cell autonomous; (4) be cell localized; (5) exist in variant forms which are selectively neutral; (6) have variant forms which can be detected in histologic sections; and (7) have variants which are expressed early in embryogenesis.

Keywords

Cell Marker Marker System Bergmann Glial Cell Cellulose Phosphate Mouse Chimera 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Askenase, P. W. and E. J. Leonard. 1970. Solid phase radioimmunoassay of human β-1C globulin. Immunochem. 7: 29–41.CrossRefGoogle Scholar
  2. Chapman, V. M., W. K. Whitten and F. H. Ruddle. 1971. Expression of paternal glucosephosphate isomerase-1 (GPI-1) in preimplantation mouse embryos. Devel. Biol. 26: 153–158.CrossRefGoogle Scholar
  3. Condamine, H., R. P. Custer and B. Mintz. 1971. Pure-strain and genetically mosaic liver tumors histochemically identified with the β-glucuronidase marker in allophenic mice. Proc. Nat. Acad. Sci., USA 68: 2032–2036.CrossRefGoogle Scholar
  4. Cuatrecasas, P. 1970. Protein purification by affinity chromatography. Derivations of agarose and Polyacrylamide beads. J. Biol. Chem. 245: 3059–3065.PubMedGoogle Scholar
  5. DeLorenzo, R. J. and F. H. Ruddle. 1969. Genetic control of two electrophoretic variants of glucosephosphate isomerase in the mouse (Mus musculus). Biochem. Genet. 3: 151–162.PubMedCrossRefGoogle Scholar
  6. Dewey, M. J., A. G. Gervais and B. Mintz. 1976. Brain and ganglion development from two genotypic classes of cells in allophenic mice. Devel. Biol. 50: 68–81.CrossRefGoogle Scholar
  7. Feder, N. 1976. Solitary cells and enzyme exchange in tetraparental mice. Nature 263: 67–69.PubMedCrossRefGoogle Scholar
  8. Gearhart, J. D. and B. Mintz. 1972. Clonal origins of somites and their muscle derivatives: Evidence from allophenic mice. Devel. Biol. 29: 27–37.CrossRefGoogle Scholar
  9. Gearhart, J. and M. L. Oster-Granite. 1978. Isolation of glucosephosphate isomerase from mouse tissues and the obtainment of anti-GPI antisera. Submitted for publication.Google Scholar
  10. Herrup, K. and R. J. Mullen. 1977. Biochemical and genetic factors in the heat inactivation of murine-β-glucuronidase. Biochem. Genet. 15: 641–653.PubMedCrossRefGoogle Scholar
  11. Hunter, W. M. and F. C. Greenwood, 1962. Preparation of iodine-131 labeled human growth hormone of high specific activity. Nature 194: 495–496.PubMedCrossRefGoogle Scholar
  12. McLaren, A. 1976. Mammalian Chimaeras. Cambridge University Press, Cambridge, Great Britain.Google Scholar
  13. Mintz, B. 1974. Gene control of mammalian differentiation. Ann. Rev. Genet. 8: 411–470.PubMedCrossRefGoogle Scholar
  14. Mullen, R. J. 1977. Genetic dissection of the CNS with mutant-normal mouse and rat chimeras, In Soc. Neurosci. Symp. 2: 47–65. W. M. Cowan and J. A. Ferendelli, eds.Google Scholar
  15. Mullen, R. J. 1978. Presented at this conference.Google Scholar
  16. Padua, R. A., G. Bulfield and J. Peters. 1978. Biochemical genetics of a new glucosephosphate isomerase allele (Gpi-1 c ) from wild mice. Biochem. Genet. 16: 127–143.PubMedCrossRefGoogle Scholar
  17. Phillips, T. L., J. M. Talent and R. W. Gracy. 1976. Isolation of rabbit glucosephosphate isomerase by a single-step substrate elution. Biochem. Biophys. Acta 429: 624–628.PubMedGoogle Scholar
  18. Rodriguez, J. and F. Deinhardt. 1960. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology 12: 316–317.PubMedCrossRefGoogle Scholar
  19. Sainte-Marie, G. 1962. A paraffin embedding technique for studies employing immunofluorescence. J. Histochem. Cytochem. 10: 250–256.CrossRefGoogle Scholar
  20. Swank, R. T., K. Paigen and R. E. Ganschow. 1974. Genetic control of glucuronidase induction in mice. J. Mol. Biol. 81: 225–243.CrossRefGoogle Scholar
  21. Yellin, H. and L. Guth. 1970. The histochemical classification of muscle fibers. Exper. Neurol. 26: 424–432.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • John Gearhart
    • 1
  • Mary Lou Oster-Granite
    • 1
  1. 1.Department of Anatomy, School of MedicineUniversity of MarylandBaltimoreUSA

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