Preferential Inactivation of the Paternally Derived X Chromosome in Mice

  • Nobuo Takagi
Part of the Basic Life Sciences book series (BLSC, volume 12)


Random X-chromosome inactivation makes the female of placental mammals a natural mosaic for clones of cells having either the maternally derived X (Xm) or paternally derived one (XP) genetically inactivated (Lyon 1961). In marsupials, on the other hand, preferential inactivation of the paternally inherited X chromosome seems to be a rule in most tissues (Sharman 1971; Cooper, Johnston, Murtagh, Sharman, VandeBerg, and Poole 1975). There are, however, instances in which inactivation is obviously not random in placental mammals (Lyon 1974). Nonrandomness was assessed from studies made on differentiated cells remote from early embryonic cells in which inactivation occurred. Thus it is not clear whether nonrandom inactivation, or a secondary event(s) after random inactivation, was responsible for the ultimate nonrandom expression.


Primitive Endoderm Preimplantation Mouse Embryo Embryonic Region Extraembryonic Mesoderm Random Inactivation 
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  1. Barlow, P. W., D. Owen and C. F. Graham. 1972. DNA synthesis in the preimplantation mouse embryo. J. Embryol. exp. Morph. 27: 431–445.PubMedGoogle Scholar
  2. Cattanach, B. M. 1961. A chemically-induced variegated-type position effect in the mouse. Z. VererbLehre 92: 165–182.Google Scholar
  3. Cattanach, B. M. 1975. Control of chromosome inactivation. Ann. Rev. Genet. 9: 1–18.PubMedCrossRefGoogle Scholar
  4. Cattanach, B. M. and C. E. Williams. 1972. Evidence of nonrandom X chromosome activity in the mouse. Genet. Res. 19: 229–240.PubMedCrossRefGoogle Scholar
  5. Cooper, D. W. 1971. Directed genetic change model for X chromosome inactivation in eutherian mammals. Nature, Lond. 230: 292–294.CrossRefGoogle Scholar
  6. Cooper, D. W., P. G. Johnston, C. E. Murtagh, G. B. Sharman, J. L. VandeBerg and W. E. Poole. 1975. In Isozymes. Vol. III. Developmental Biology, C. L. Markert, Ed., pp. 559–573. San Francisco: Academic Press.Google Scholar
  7. Drews, U., S. R. Blecher, D. A. Owen and S. Ohno. 1974. Genetically directed preferential X-activation seen in mice. Cell 1: 3–8.CrossRefGoogle Scholar
  8. Dutrillaux, B., C. Laurent, J. Couturier and J. Lejeune. 1973. Coloration des chromosomes humains par l’acridine orange après traitement par le 5 bromodeoxyuridine. C. R. Acad. Sc. Paris 276: 3179–3181.Google Scholar
  9. Gardner, R. L. and M. H. Johnson. 1975. Investigation of cellular interaction and development in the early mammalian embryo using interspecific chimaeras between the rat and mouse. In Cell Patterning (Ciba Found. Symp. 29), pp. 183–200. Amsterdam: Associated Scientific Publishers.Google Scholar
  10. Gardner, R. L. and M. F. Lyon. 1971. X chromosome inactivation studied by injection of a single cell into the mouse blastocyst. Nature, Lond. 231: 385–386.CrossRefGoogle Scholar
  11. Gardner, R. L. and V. E. Papaioannou. 1975. Differentiation in the trophectoderm and inner cell mass. In The Early Development of Mammals, pp. 107–132. London: Cambridge University Press.Google Scholar
  12. Hoppe, P. C. and K. Illmensee. 1977. Microsurgically produced homozygous-diploid uniparental mice. Proc. Natl. Acad. Sci. USA 74: 5657–5661.PubMedCrossRefGoogle Scholar
  13. Lyon, M. F. 1961. Gene action in the X-chromosome of the mouse (Mus musculus L.). Nature, Lond. 190: 372–373.CrossRefGoogle Scholar
  14. Lyon, M. F. 1974. Mechanisms and evolutionary origins of variable X-chromosome activity in mammals. Proc. roy. Soc. B. 187: 243–268.CrossRefGoogle Scholar
  15. Monk, M. 1978. Biochemical studies on X-chromosome activity in preimplantation mouse embryos. In Symposium on Genetic Mosaics and Chimeras in Mammals, Liane B. Russell, Ed. Plenum Press, New York and London.Google Scholar
  16. Monk, M. and Kathuria, H. 1977. Dosage compensation for an X-linked gene in preimplantation mouse embryos. Nature, Lond. 270: 599–601.CrossRefGoogle Scholar
  17. Nielsen, J. T. and V. M. Chapman. 1977. Electrophoretic variation for sex-linked phosphoglycerate kinase (PGK-1) in the mouse. Genetics 87: 319–325.PubMedGoogle Scholar
  18. Ohno, S., L. N. Geller and J. Kan. 1974. The analysis of Lyon’s hypothesis through preferential X-activation. Cell 1: 175–184.CrossRefGoogle Scholar
  19. Rattazzi, M. C. and M. M. Cohen. 1971. Further proof of genetic inactivation of the X-chromosome in the female mule. Nature, Lond. 237: 393–395.CrossRefGoogle Scholar
  20. Sharman, G. B. 1971. Late DNA replication in the paternally derived X chromosome of female kangaroos. Nature, Lond. 230: 231–232.CrossRefGoogle Scholar
  21. Snell, G. D. and L. C. Stevens. 1966. Early embryology. In Biology of the Laboratory Mouse, 2nd edition, E. L. Green, Ed. New York: McGraw-Hill.Google Scholar
  22. Takagi, N. 1974. Differentiation of X chromosomes in early female mouse embryos. Exptl. Cell Res. 86: 127–135.PubMedCrossRefGoogle Scholar
  23. Takagi, N. 1976. Stability of X chromosome differentiation in mouse embryos: reversal may not be responsible for the extreme X-inactivation mosaicism in extraembryonic membranes. Hum. Genet. 34: 207–211.PubMedCrossRefGoogle Scholar
  24. Takagi, N. and M. Oshimura. 1973. Fluorescence and Giemsa banding studies of the allocyclic X chromosome in embryonic and adult mouse cells. Exptl. Cell Res. 78: 127–135.PubMedCrossRefGoogle Scholar
  25. Takagi, N. and Sasaki, M. 1975. Preferential inactivation of the paternally derived X chromosome in the extraembryonic membranes of the mouse. Nature, Lond. 256: 640–642.CrossRefGoogle Scholar
  26. Takagi, N., N. Wake and M. Sasaki. 1978. Cytologic evidence for preferential inactivation of the paternally derived X chromosome in XX mouse blastocysts. Cytogenet. Cell Genet. 20: 240–248.PubMedCrossRefGoogle Scholar
  27. Wake, N., N. Takagi and M. Sasaki. 1976. Nonrandom inactivation of X chromosome in the rat yolk sac. Nature, Lond. 262: 580–581.CrossRefGoogle Scholar
  28. West, J. D., W. I. Frels, V. M. Chapman and V. E. Papaioannou. 1977. Preferential expression of the maternally derived X chromosome in the mouse yolk sac. Cell 12: 873–882.PubMedCrossRefGoogle Scholar
  29. Wroblewska, J. and A. P. Dyban. 1969. Chromosome preparation from embryos during early organogenesis: Dissociation after fixation, followed by air drying. Stain Technol. 44: 147–150.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Nobuo Takagi
    • 1
  1. 1.Chromosome Research Unit, Faculty of ScienceHokkaido UniversitySapporoJapan

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