I would like to comment on the paper by Dr. Cunningham-Rundles. Although the data presented strongly suggests that milk antigens were involved in producing immune complexes, you need more substantial proof. We have just recently published a technique in the Journal of Immunological Methods that, I think, would be applicable. It is based on precipitation of the immune complexes with PEG-PEG, their separation on Sephacryl 34 column, (complexes are eluted with the exclusion peak) and their further purification with the use of protein A. From protein A, antigen and antibody can be eluted and characterized.
KeywordsImmune Complex Human Milk Coeliac Disease Pernicious Anemia Lichen Planus
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