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Discussion

  • Jerry R. McGhee
  • Jiri Mestecky
  • James L. Babb
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 107)

Abstract

I would like to discuss some of our findings that concern measurements of lysozyme. I agree with Dr. Cole that with freshly collected samples, one can get much more reliable results, but this still does not prevent two problems. Measurements of low concentrations of lysozyme on the lysoplate can be rather difficult because the lysis rings are not that sharp. The second problem concerns the day to day variation of the lysoplate which runs in the 15% range. Therefore, when you are measuring low amounts with these kinds of variables, your estimates are not very precise. Acidification of the samples allows you to determine lysozyme levels by nephelometry with anti-lysozyme serum. This method is far more accurate. I think this is what you should do when you are determining really low levels of lysozyme in saliva. The other point is that acidification does not harm the immunoglobulins. You can run unconcentrated saliva in the nephelometer with commercial standards and get determination of immunoglobulin values right away. If you consider the errors you make, in concentrating saliva samples, and in radial immunodiffusion because of different molecular sizes of IgA, I think you will have to agree with me that it is better to do the measurements by nephelometry.

Keywords

Radial Immunodiffusion Oral Flora Parotid Saliva High Molecular Weight Substance Lactoferrin Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Jerry R. McGhee
    • 1
  • Jiri Mestecky
    • 1
  • James L. Babb
    • 1
  1. 1.Institute of Dental Research, Departments of Microbiology and Medicine, Comprehesive Cancer CenterUniversity of Alabama in BirminghamBirminghamUSA

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