Antibody Binding of Glucosyltransferase Enzyme Preparations from Homologous and Heterologous Serotypes of S. Mutans
The virulence of Streptococcus mutans with respect to dental caries is related to extracellular glucose polymers synthesized from sucrose (1) by a group of constitutive enzymes called glucosyltransferase (GTF) (2). Recent studies, in which rodents were immunized with GTF enzymes of varying degrees of purity, have indicated that antibody directed to GTF might be protective with respect to dental caries caused by the same organisms (3). Although there are at least 7 serotypes of S. mutans (4), studies of in vitro GTF functional inhibition (5,6) indicate that GTF ethanol-insoluble polysaccharide synthesizing activity from serotypes ji or c[or £ can be inhibited by antisera directed to either GTF. Similarly, GTF from serotypes c or e, and possibly b, are also inhibitable by antisera directed to either c, e or b GTF (homologous). However, antisera directed to GTF of either subset (c, e, b or a, d, g) show minimal inhibition of total glucan (ethanol-insoluble) synthesized by the heterologous subset of GTF (6). Despite these findings it is conceivable that antibody can bind to GTF of the heterologous subset and can thus be protective with respect to an infection with an S. mutans strain synthesizing such heterologous GTF. The ability of antibody directed to GTF to bind heterologous GTF has been explored in this investigation.
KeywordsDental Caries GLUCOSYL Transferase Salivary Antibody Heterologous SEROTYPES Relative Elution Volume
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