Cellular Parameters of the IgA Response
Peyer’s patches (PP) and bronchial lymphoid follicles of a number of mammals have been shown to be reservoirs of B-lymphocytes with a special potential to generate IgA plasma cells (1–4). These observations led us to postulate that PP cells may encounter antigen in situ and be clonally expanded (4, 5). Following cell division some progeny may become motile and emigrate via the mesenteric lymph nodes (MLN) and thoracic duct, lymph (TDL) into the circulation. Dependent upon cellular and humoral interactions enroute these cells may divide and differentiate to varying extents. For instance, cells may mature enroute to plasmablasts with cytoplasmic IgA followed by selective lodging in secretory tissue, respiratory mucosa or gastrointestinal mucosa. Alternatively, IgA precursors may lodge in mucosal tissue relatively soon after departing the PP, divide there further, and give rise to some progeny which express cytoplasmic IgA. Support for this scheme requires knowledge of the antigen-sensitivity of PP cells, their potential to generate IgA producing progeny, the cellular and humoral factors which control their differentiation and expression of products, and their ability to selectively populate distant mucosae. We have approached these matters using variations of the adoptive transfer system. Either the transferred cells were left in their host to manifest their potential or the recipients’ spleens were removed shortly after transfer, when they had collected a sample of the transferred cells, and used for clonal analysis of the potential of these cells.
KeywordsFractionation Inulin Ascaris Phosphocholine Dinitrophenyl
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