Remote-Site Stimulation of Secretory IgA Antibodies Following Bronchial and Gastric Stimulation
The induction of secretory antibodies remains a complex subject with a variety of factors being important: 1) choice of the animal model, 2) the nature of the antigenic stimulus and 3) the method of immunization. Our own investigations have utilized the pregnant rabbit and various DNP-carrier conjugates to probe the manner by which secretory antibodies, particularly secretory (s) IgA, were induced in mammary glands (1–3). These studies demonstrated that: 1) local (intramammary) administration of antigen was effective at reproducibly eliciting s-IgA antibodies (confirming earlier studies (4,5), 2) there was a lack of correlation between the induction of high levels of s-IgA antibodies and serum IgG antibodies, 3) there appeared to be a special relationship between gastrointestinal stimulation and remote-site (mammary) production of s-IgA antibodies, and 4) gastrointestinal stimulation of s-IgA antibodies in milk was not blocked by high levels of circulating IgG serum antibodies. Additional evidence suggested that a similar “remote-site” stimulation phenomenon also existed in other animal species.
KeywordsSecretory Antibody Gastric Stimulation Solid Phase Radioimmunoassay Mesenteric Lymph Node Cell Pilocarpine Hydrochloride
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