Remote-Site Stimulation of Secretory IgA Antibodies Following Bronchial and Gastric Stimulation

  • Paul C. Montgomery
  • Kevin M. Connelly
  • Judith Cohn
  • Cheryl A. Skandera
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 107)


The induction of secretory antibodies remains a complex subject with a variety of factors being important: 1) choice of the animal model, 2) the nature of the antigenic stimulus and 3) the method of immunization. Our own investigations have utilized the pregnant rabbit and various DNP-carrier conjugates to probe the manner by which secretory antibodies, particularly secretory (s) IgA, were induced in mammary glands (1–3). These studies demonstrated that: 1) local (intramammary) administration of antigen was effective at reproducibly eliciting s-IgA antibodies (confirming earlier studies (4,5), 2) there was a lack of correlation between the induction of high levels of s-IgA antibodies and serum IgG antibodies, 3) there appeared to be a special relationship between gastrointestinal stimulation and remote-site (mammary) production of s-IgA antibodies, and 4) gastrointestinal stimulation of s-IgA antibodies in milk was not blocked by high levels of circulating IgG serum antibodies. Additional evidence suggested that a similar “remote-site” stimulation phenomenon also existed in other animal species.


Secretory Antibody Gastric Stimulation Solid Phase Radioimmunoassay Mesenteric Lymph Node Cell Pilocarpine Hydrochloride 
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  1. 1.
    Montgomery, P. C., Rosner, B. R. and Cohn, J., Immunol. Comm. 3: 143, 1974.Google Scholar
  2. 2.
    Montgomery, P. C., Cohn, J. and Lally, E. T., in The Immunoglobulin A System (Edited by Mestecky, J. and Lawton, A. R.), p. 453, Plenum Press, New York, 1974.CrossRefGoogle Scholar
  3. 3.
    Montgomery, P. C., Lemaitre-Coelho, I. and Lally, E. T., La Ricerca 6(Suppl. 3): 93, 1976.Google Scholar
  4. 4.
    Genco, R. J. and Taubman, M. A., Nature 221: 679, 1969.PubMedCrossRefGoogle Scholar
  5. 5.
    Taubman, M. A. and Genco, R. J., Immunochemistry 8: 1137, 1971.PubMedCrossRefGoogle Scholar
  6. 6.
    Allardyce, R. A., Shearman, D.J.C., McClelland, B.B.L., Marwick, K., Simpson, A. J. and Laidlaw, R. B., Brit. Med. J. 3: 307, 1974.PubMedCrossRefGoogle Scholar
  7. 7.
    Goldblum, R. M., Ahlstedt, S., Carlsson, B., Hanson, L. A., Jodal, V., Lindin-Janson, G. and Sohl-Akerlund, A., Nature 237: 797, 1975.CrossRefGoogle Scholar
  8. 8.
    Michalek, S. M., McGhee, J. R., Mestecky, J., Arnold, R. R. and Bozzo, L., Science 192: 1238, 1976.PubMedCrossRefGoogle Scholar
  9. 9.
    Mestecky, J., McGhee, J.R., Arnold, R. R., Michalek, S. M., Prince, S. J. and Babb, J. L., J. Clin. Invest. 1978.Google Scholar
  10. 10.
    Little, J. R. and Eisen, H. N. , in Methods in Immunology and Immunochemistry (Edited by Williams, C. A. and Chase, M. W.), Vol. 1, p. 128, Academic Press, New York, 1967.Google Scholar
  11. 11.
    Montgomery, P. C. and Pincus, J. H., J. Immunol. 111: 42, 1973.PubMedGoogle Scholar
  12. 12.
    Montgomery, P. C. and Williamson, A. R., J. Immunol. 109: 1036, 1972.PubMedGoogle Scholar
  13. 13.
    Cebra, J. J. and Robbins, J. B., J. Immunol. 97, 12, 1966.PubMedGoogle Scholar
  14. 14.
    Robbins, J. B., Haimovich, J. and Cela, M., Immunochemistry 4: 11, 1967.PubMedCrossRefGoogle Scholar
  15. 15.
    Hunter, W. M. and Greenwood, F. G., Nature 194: 495, 1962.PubMedCrossRefGoogle Scholar
  16. 16.
    Klinman, N. R. and Taylor, R. B., Clin. Exp. Immunol. 4: 473, 1969.PubMedGoogle Scholar
  17. 17.
    Askenase, P. W. and Leonard, E. J., Immuno chemistry 7: 29, 1970.CrossRefGoogle Scholar
  18. 18.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., J. Biol. Chem. 193: 265, 1951.PubMedGoogle Scholar
  19. 19.
    Heremans, J.F., in The Immunoglobulin A System (Edited by Mestecky, J. and Lawton, A. R.), p. 3, Plenum Press, New York, 1974.CrossRefGoogle Scholar
  20. 20.
    Mestecky, J., La Ricera 6(Suppl. 3): 87, 1976.Google Scholar
  21. 21.
    McWilliams, M., Philips-Quagliata, J. M., Lamm, M. E., J. Immunol. 115: 54, 1975.PubMedGoogle Scholar
  22. 22.
    Roux, M. E., McWilliams, M., Philips-Quagliata, J. M., Weisz-Carrington, P. and Lamm, M. E., J. Exp. Med. 146: 1311, 1977.PubMedCrossRefGoogle Scholar
  23. 23.
    Rudzik, R., Clancy, R. L., Perey, D.Y.E., Day, R. P. and Bienenstock, J., J. Immunol. 114: 1599, 1975PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Paul C. Montgomery
    • 1
  • Kevin M. Connelly
    • 1
  • Judith Cohn
    • 1
  • Cheryl A. Skandera
    • 1
  1. 1.Department of Microbiology, School of Dental Medicine and Center for Oral Health ResearchUniversity of PennsylvaniaPhiladelphiaUSA

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