Amino Acid Supplementation of Isolated Soybean Protein in Milk Replacers for Preruminant Lambs

  • Rodrigo Pelaez
  • David D. Phillips
  • Donald M. Walker
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 105)


The growth of preruminant calves and lambs fed on milk replacers containing vegetable proteins is generally inferior to that of preruminants given cows’ milk. The inferior performance has variously been attributed to an amino acid imbalance, to heat damage in preparation, and to the presence of growth-retarding substances. Soybean products that have been treated to remove, or destroy, growth-retarding substances are now available commercially.

An experimental design is described that has been used to determine the order of limiting amino acids in soy protein isolates, and which may also be used to estimate requirements for individual amino acids. The design allows an economy in time and in experimental animals, and gives results that are not significantly different from those determined in classical balance experiments.


Nitrogen Balance Soybean Protein Vegetable Protein Amino Acid Supplementation Limit Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Benevenga, N.J. and Ronning, M. (1963). Response of calves to high fat milk replacer diets. J. Anim. Sci., 22, 832.Google Scholar
  2. Berry, T.H., Becker, D.E., Rasmussen, O.G., Jensen, A.H. and Norton, H.W. (1962). The limiting amino acids in soybean protein. J. Anim. Sci., 21, 558–561.Google Scholar
  3. Blaxter, K.L. and Wood, W.A. (1953). Some observations on the biochemical and physiological events associated with diarrhoea in calves. Vet. Rec., 65, 889–892.Google Scholar
  4. Brown, J.A. and Cline, T.R. (1974). Urea excretion in the pig: an indicator of protein quality and amino acid requirements. J. Nutr., 104, 542–545.Google Scholar
  5. Das, T.K. and Waterlow, J.C. (1974). The rate of adaptation of urea cycle enzymes, aminotransferasesand glutamic dehydrogenase to changes in dietary protein intake. Br. J. Nutr., 32, 353–373.CrossRefGoogle Scholar
  6. Eggum, B.O. (1970). Blood urea measurement as a technique for assessing protein quality. Br. J. Nutr., 24, 983–988.CrossRefGoogle Scholar
  7. Gertler, A., Birk, Y. and Bondi, A. (1967). A comparative study of the nutritional and physiological significance of pure soybean trypsin inhibitors and of ethanol-extracted soybean meals in chicks and rats. J. Nutr., 91, 358–370.Google Scholar
  8. Gorrill, A.D.L. and Nicholson, J.W.G. (1969). Growth, digestibility and nitrogen retention by calves fed milk replacers containing milk and soybean proteins, supplemented with methionine. Canad. J. Anim. Sci., 49, 315–321.CrossRefGoogle Scholar
  9. Gorrill, A.D.L. and Nicholson, J.W.G. (1971). Effect of soybean trypsin inhibitor, diarrhoea and diet on flow rate, pH, proteolytic enzymes, and nitrogen fractions in calf intestinal digesta. Canad. J. Anim. Sci., 51, 377–388.CrossRefGoogle Scholar
  10. Gorrill, A.D.L. and Nicholson, J.W.G. (1972). Alkali treatment of soybean protein concentrate in milk replacers: its effects on digestion, nitrogen retention, and growth of lambs. Canad. J. Anim. Sci., 52, 665–670.CrossRefGoogle Scholar
  11. Huber, J.T. (1969). Development of the digestive and metabolic apparatus of the calf. J. Dairy Sci., 52, 1303–1315.CrossRefGoogle Scholar
  12. Kirk, R.D. and Walker, D.M. (1976a). Plasma urea nitrogen as an indicator of protein quality. I. Factors affecting the concentration of urea in the blood of the preruminant lamb. Aust. J. Agric. Res., 27, 109–116.CrossRefGoogle Scholar
  13. Kirk, R.D. and Walker, D.M. (1976b). Plasma urea nitrogen as an indicator of protein quality. II. Relationships between plasma urea nitrogen, various urinary nitrogen constituents, and protein quality. Aust. J. Agric. Res., 27, 117–127.CrossRefGoogle Scholar
  14. Mattil, K.F. (1974). Composition, nutritional, and functional properties, and quality criteria of soy protein concentrates and soy protein isolates. J. Am. Oil Chemists Soc., 51, 81A - 84A.CrossRefGoogle Scholar
  15. Mickelsen, O. and Yang, M.G. (1966). Naturally occurring toxicants in foods. Fed. Proc., 25, 104–123.Google Scholar
  16. Nitsan, Z., Volcani, R., Gordin, S. and Hasdai, A. (1971). Growth and nutrient utilization by calves fed milk replacers containing milk or soybean protein-concentrate heated to various degrees. J. Dairy Sci., 54, 1294–1299.CrossRefGoogle Scholar
  17. Nitsan, Z., Volcani, R., Hasdai, A. and Gordin, S. (1972). Soybean protein substitute for milk protein in milk replacers for suckling calves. J. Dairy Sci., 55, 811–821.CrossRefGoogle Scholar
  18. Orr, M.L. and Watt, B.K. (1968). Amino acid content of foods. U.S. Dep. Agric. Home Econ. Res. Rep. No. 4. U.S. Govt. Printing Office, Washington.Google Scholar
  19. Patterson, H.D. and Lucas, H.L. (1962). Change-over Designs. Tech. Bull. no. 147. North Carolina Agric. Expt. Sta.Google Scholar
  20. Porter, J.W.G. (1969). Digestion in the preruminant animal. Proc. Nutr. Soc., 28, 115–121.CrossRefGoogle Scholar
  21. Smith, R.H. and Sissons, J.W. (1975). The effect of different feeds, including those containing soya-bean products, on the passage of digesta from the abomasum of the preruminant calf. Br. J. Nutr., 33, 329–349.CrossRefGoogle Scholar
  22. Snetsinger, D.C. and Scott, H.M. (1958). The adequacy of soybean oil meal as a sole source of protein for chick growth. Poultry Sci., 37, 1400–1403.CrossRefGoogle Scholar
  23. van Adrichem, P.W.M. and Frens, A.M. (1965). Soya protein as a feed antigen for fattening calves. Tijdschr. Diergeneesk., 90, 525–530.Google Scholar
  24. van Leeuwen, J.M. Weide, H.J. and Braas, C.C. (1969). Feeding value of soyabean oilmeal compared with dried skimmed milk. Versl. landbouwk. Onderz. Ned., no. 732.Google Scholar
  25. Walker, D.M. (1959). The development of the digestive system of the young animal. III. Carbohydrase enzyme development in the young lamb. J. Agric. Sci., 53, 374–386.CrossRefGoogle Scholar
  26. Walker, D.M. (1975). Utilization of whole egg and its components in milk replacers for preruminant lambs. Aust. J. Agric. Res., 26, 599–614.CrossRefGoogle Scholar
  27. Walker, D.M. and Faichney, G.J. (1964). Nutritional diarrhoea in the milk-fed lamb and its relation to the intake of sugar. Br. J. Nutr., 18, 209–215.CrossRefGoogle Scholar
  28. Walker, D.M. and Kirk, R.D. (1975a). The utilization by pre-ruminant lambs of milk replacers containing isolated soya bean protein. Aust. J. Agric. Res., 26, 1025–1035.CrossRefGoogle Scholar
  29. Walker, D.M. and Kirk, R.D. (1975b). The utilization by pre-ruminant lambs of isolated soya bean protein in low protein milk replacers. Aust. J. Agric. Res., 26, 1037–1052.CrossRefGoogle Scholar
  30. Yoshida. A., Umai, A., Kurata, Y. and Kawamura, S. 1969). Utilization of soybean oligosaccharides by the intact rat. J. Jap. Soc. Fd. Nurt., 22, 262–265. (quoted in Nutr. Abstr Rev., 1970, 40, Abstr. 676).Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Rodrigo Pelaez
    • 1
  • David D. Phillips
    • 1
  • Donald M. Walker
    • 1
  1. 1.Department of Animal HusbandryUniversity of SydneySydneyAustralia

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