Advertisement

Short-Term Unilateral Ischemia in Gerbils: A Reevaluation

  • N. Murakami
  • W. D. Lust
  • A. B. Wheaton
  • J. V. Passonneau

Abstract

Our previous studies on unilateral and bilateral ischemia in the gerbil have been primarly concerned with the sites directly affected by the loss of circulation. In these regions, the changes in brain metabolites, including energy reserves and cyclic nucleotides, have been pronounced (4, 8). In earlier studies with unilateral ischemia, there were little or no observable changes in these metabolites in the contralateral cortex (8, 11). However, Levy and Duffy (6) have recently demonstrated that there are changes not only in the contralateral cortex of affected gerbils but also in the ipsilateral cortex of animals not exhibiting positive neurological signs of ischemia, a condition which was completely ignored in our previous studies. It appears that the biochemical effects of an ischemic episode are variable depending upon the region of the brain investigated and perhaps on the neurological responses elicited. The intermediate range of responses in those areas not directly affected by the carotid artery occlusion is the subject of this paper.

Keywords

Common Carotid Artery Neurological Sign Carotid Artery Occlusion Ischemic Episode Brain Metabolite 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Ferrendelli, J.A. and McDougal, D.B. (1971): The effect of electroshock on regional CNS energy reserves in mice J. Neurochem. 18: 1197–1208.PubMedCrossRefGoogle Scholar
  2. 2.
    Kahn, K. (1972): The natural course of experimental cerebral infarction in the gerbil. Neurology, 22: 510–515.PubMedGoogle Scholar
  3. 3.
    King, L.J., Lowry, O.H., Passonneau, J.V. and Venson, V. (1967): Effects of convulsants on energy reserves in the cerebral cortex. J. Neurochem. 14: 599–611.PubMedCrossRefGoogle Scholar
  4. 4.
    Kobayashi, M., Lust, W.D. and Passonneau, J.V.(1977): Concentrations of energy metabolites and cyclic nucleotides during and after bilateral ischemia in the gerbil cerebral cortex. J. Neurochem. 29: 53–59.PubMedCrossRefGoogle Scholar
  5. 5.
    Levine, S. (1960): Anoxic-ischemic encephalopathy in rats. Amer. J. Path. 36: 1–15.PubMedGoogle Scholar
  6. 6.
    Levy, D.E. and Duffy, T.E. (1977): Cerebral energy metabolism during transient ischemia and recovery in the gerbil. J. Neurochem. 28: 63–70.PubMedCrossRefGoogle Scholar
  7. 7.
    Lowry, O.H. and Passonneau, J.V. (1972): In A Flexible System of Enzymatic Analysis. Academic Press, New York 151–156.Google Scholar
  8. 8.
    Lust, W.D., Mršulja, B.B., Mršulja, B.J., Passoneau, J.V. and Klatzo, I. (1975): Putative neurotransmitters and cyclic nucleotides in prolonged ischemia of the cerebral cortex. Brain Research, 98: 394–399.PubMedCrossRefGoogle Scholar
  9. 9.
    Lust, W.D. and Passonneau, J.V. (1976): Cyclic nucleotides in murine brain: Effect of hypothermia on cyclic AMP, glycogen Phosphorylase, glycogen synthase and metabolites following maximal electroshock or decapitation. J. Neurochem. 26: 11–16.PubMedCrossRefGoogle Scholar
  10. 10.
    McGraw, C.P. (1977): Experimental cerebral infarctation effects of phenobarbital in mongolian gerbils. Arch. Neurol. 34: 334–336.PubMedCrossRefGoogle Scholar
  11. 11.
    Mršulja, B.B., Lust, W.D., Mršulja, B.J., Passonneau, J.V. and Klatzo, I. (1976): Post-ischemic changes in certain metabolites following prolonged ischemia in the gerbil cerebral cortex. J. Neurochem. 26: 1099–1103.CrossRefGoogle Scholar
  12. 12.
    Passonneau, J.V. and Lauderdale, V.R. (1974): A comparison of three methods of glycogen measurement in tissues. Anal. Biochem. 60: 405–512.PubMedCrossRefGoogle Scholar
  13. 13.
    Smith, A.L. and Wollman, H. (1972): Cerebral blood flow and metabolism: Effects of anesthesia drugs and techniques. Anesthesiology. 36: 378–400.PubMedCrossRefGoogle Scholar
  14. 14.
    Steiner, A.L., Wehmann, R.E., Parker, C.W. and Kipnis, D.M. (1972): Radioimmunoassay for the measurement of cyclic nucleotides. In Advances in Cyclic Nucleotide Research. (Greengard, P. and Robinson, G.A., eds.). Raven Press, New York, 2: 51–61.Google Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • N. Murakami
    • 1
  • W. D. Lust
    • 1
  • A. B. Wheaton
    • 1
  • J. V. Passonneau
    • 1
  1. 1.Laboratory of Neurochemistry, National Institute of Neurological and Communicative Disorders and StrokeNational Institutes of HealthBethesdaUSA

Personalised recommendations