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Cytochemical Staining Reactions for Enzymes in Cytoplasmic Organelles

  • Alex B. Novikoff
  • Phyllis M. Novikoff

Abstract

Recent reports on the use of lead precipitating procedures for localizing intracellular phosphatases and criticism of these procedures (see (1) and (2) for the most recent interchange of views) have left undiminished the value of using the following phosphatases as cytochemical “markers” for light and electron microscopy (3, 4): plasma membrane — nucleoside phosphatases (e.g., Mn++ — and Mg++-stimulated “ATPase”, 5’-nucleotidase, Co++-stimulated CMPase, etc.); endoplasmic reticulum (ER) of liver, kidney, endocrine-secreting cells, etc. -- a nucleoside diphosphatase (NDPase) that hydrolyzes IDP, UDP, GDP (also TPP) but not CDP or ADP (5, 6); Golgi apparatus -- NDPase, thiamine pyrophosphatase (TTPase) (see 6)); Golgi-endoplasmic reticulumlysosome (GERL) -- acid phosphatase; and lysosomes -- acid phosphatase. Optimal oxidation at high pH and relatively high H2O2 concentration of diaminobenzidine may be used to “mark” peroxisomes because of their high catalase content (7, 8). Mitochondria oxidize diaminobenzidine optimally at low pH and low H2O2 concentration (7).

Keywords

Dorsal Root Ganglion Golgi Apparatus Smooth Endoplasmic Reticulum Glycosyl Transferase Lead Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Rosenthal, A.S., Moses, H.L. and Ganote, C.E., Interpretation of Phosphatase Cytochemical Data. J. Histochem. Cytochem., 18: 915, 1970.PubMedCrossRefGoogle Scholar
  2. 2.
    Novikoff, A.B., Their Phosphatase Controversy: Love’s Labour’s Lost. J. Histochem. Cytochem., 18: 916–917, 1970.PubMedCrossRefGoogle Scholar
  3. 3.
    Novikoff, A.B., Membrane-Bound Enzymes. Abstracts, Sixth Intl. Congress of Biochemistry, New York, VIII-S4, p. 609, 1964.Google Scholar
  4. 4.
    Novikoff, A.B., Essner, E., Goldfischer, S. and Heus, M., Nucleosidephosphatase Activities of Cytomembranes. Symp. Intl. Soc. Cell Biol., 1:149–192, 1962. [The Interpretation of Ultrastructure (R.J.C. Harris, ed.), Academic Press, N.Y.]Google Scholar
  5. 5.
    Novikoff, A.B. and Heus, M., A Microsomal Nucleoside Diphosphatase. J. Biol. Chem., 238: 710–716, 1963.PubMedGoogle Scholar
  6. 6.
    Yamazaki, M. and Hayaishi, O., Allosteric Properties of Nucleoside Diphosphatase and its Identity with Thiamine Pyrophosphatase. J. Biol. Chem., 243: 2934, 1968.PubMedGoogle Scholar
  7. 7.
    Novikoff, A.B. and Goldfischer, S., Visualization of Peroxisomes (Microbodies) and Mitochondria with Diaminobenzidine. J. Histochem. Cytochem., 17: 675–680, 1969.Google Scholar
  8. 8.
    Beard, M.E. and Novikoff, A.B., Distribution of Peroxisomes (Microbodies) in the Nephron of the Rat. J. Cell Biol., 42: 501–518, 1969.PubMedCrossRefGoogle Scholar
  9. 9.
    Novikoff, P.M., Novikoff, A.B., Quintana, N. and Hauw, J.-J., Golgi Apparatus, GERL and Lysosomes of Neurons in Rat Dorsal Root Ganglia Studied by Thick Section and Thin Section Cytochemistry. J. Cell Biol. (in press)Google Scholar
  10. 10.
    Rambourg, A., L’appareil de Golgi examen en microscopie électronique de coupe épaisses (0.5–1 p) colorées par le mélange chlorhydrique-phosphotungstique. C. R. Acad. Sci. Paris, D 269: 2125, 1969.Google Scholar
  11. 11.
    Friend, D.S. and Murray, M., Osmium Impregnation of the Golgi Apparatus. Am. J. Anat., 117: 135, 1965.PubMedCrossRefGoogle Scholar
  12. 12.
    Fleischer, D., Fleischer, S. and Ozawa, H., Isolation and Characterization of Golgi Membranes from Bovine Liver. J. Cell Biol., 43: 59, 1969.PubMedCrossRefGoogle Scholar
  13. 13.
    Novikoff, A.B., Novikoff, P.M., Quintana, N. and Davis, C. (in preparation)Google Scholar
  14. 14.
    Novikoff, A.B., Enzyme Localization and Ultrastructure of Neurons. In: “The Neuron” ed. by H. Hyden, pp. 255–318, 1967, Elsevier Publishing Co., Amsterdam.Google Scholar
  15. 15.
    Novikoff, A.B., Albala, A. and Biempica, L., Ultra-structural and Cytochemical Observations on B-16 and Harding-Passey Mouse Melanomas: The Origin of Premelanosomes and Compound Melanosomes. J. Hlstochem. Cytochem., 16: 299–319, 1968.CrossRefGoogle Scholar
  16. 16.
    Golgi, C., Sur la Structure des Cellules Nerveuses des Ganglions Spinaux. Arch. Italiennes Biol., 30: 278–286, 1898.Google Scholar
  17. 17.
    Golgi, C., De Nouveau sur la Structure des Cellules Nerveuses des Ganglions Spinaux. Arch Italiennes Biol., 31: 273–280, 1899.Google Scholar
  18. 18.
    Novikoff, A.B. and Goldfischer, S., Nucleosidediphosphatase Activity in the Golgi Apparatus and Its Usefulness for Cytological Studies. Proceed. Natl. Acad. Sci., 47: 802–810, 1961.CrossRefGoogle Scholar
  19. 19.
    Racker, E., Burstein, C., Loyter, A. and Christiansen, R.O., The Sidedness of the Inner Mitochondrial Membrane. In: “Electron Transport and Energy Conservation” ed. by J.M. Tager, S. Papa, E. Quagliariello and E.C. Slater, pp. 235–252,1971, Adriatica Editrice, Bari, Italy.Google Scholar
  20. 20.
    Schachter, H., Jabbal, I., Hudgin, R.L., Pinteric, L., McGuire, E.J. and Roseman, S., Intracellular Localization of Liver Sugar Nucleotide Glycoprotein Glycosyltransferases in a Golgi-rich Fraction. J. Biol. Chem., 245: 1090–1100, 1970.PubMedGoogle Scholar
  21. 21.
    Novikoff, A.B., Discussion (following paper by D.W. Fawcett). Circulation. 26:1126–1129, 1962. ( Symp. on the Plasma Membrane, N.Y. Heart Association).Google Scholar
  22. 22.
    Essner, E. and Novikoff, A.B., Cytological Studies on Two Functional Hepatomas: Interrelations of Endo-plasmic Reticulum, Golgi Apparatus, and Lysosomes. J. Cell Biol., 15: 289–312, 1962.PubMedCrossRefGoogle Scholar
  23. 23.
    Marchesi, V.T. and Palade, G.E., The Localization of MgNa-K-Activated Adenosine Triphosphatase on Red Cell Ghost Membranes. J. Cell Biol., 35: 385–404, 1967.PubMedCrossRefGoogle Scholar
  24. 24.
    Novikoff, A.B. and Goldfischer, S., Visualization of Peroxisomes (Microbodies) and Mitochondria with Diaminobenzidine. J. Histochem. Cytochem., 17: 675–680, 1969.PubMedCrossRefGoogle Scholar
  25. 25.
    Beard, M.E. and Novikoff, A.B., Distribution of Peroxisomes (Microbodies) in the Nephron of the Rat. J. Cell Biol., 42: 501–518, 1969.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1971

Authors and Affiliations

  • Alex B. Novikoff
    • 1
  • Phyllis M. Novikoff
    • 1
  1. 1.Albert Einstein College of MedicineBronxUSA

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