Biomembranes pp 105-119 | Cite as

Membrane Associated Proteins

  • Sidney Fleischer
  • Warren L. Zahler
  • Hidehiro Ozawa


One approach to the study of membrane arrangement is to try to tease away membrane components and observe the effect of such treatment on ultrastructure. We found earlier that lipid can be removed from the mitochondrial inner membranes with retention of the characteristic trilaminar appearance of this membrane (1). This observation meant that the simple Dayson-Danielli model of the membrane (2) i.e., a bilayer of phospholipid sandwiched by protein could not be correct for this membrane. If so, the protein ends should collapse or become irregular when the supporting central layer was removed. However, the trilayer was retained in the absence of lipid (1). To salvage the Dayson-Danielli model supporters of this hypothesis must at least postulate that protein cross-links are present to buttress the structure.


Membrane Associate Protein Residue Fraction Rough Microsome Urea Extraction Beef Heart Mitochondrion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Fleischer, S., B. Fleischer and W. Stoeckenius. J. Cell Biol. 32, 193 (1967).PubMedCrossRefGoogle Scholar
  2. 2.
    Danielli, J. F. and H. Dayson. J. Cell Comp. Physiol. 5, 495 (1935).CrossRefGoogle Scholar
  3. 3.
    Fleischer, S., G. Rouser, B. Fleischer, A. Casu, and G. Kritchevsky. J. Lipid Res. 8, 170 (1967).PubMedGoogle Scholar
  4. 4.
    Zahler, W. L., A. Saito and S. Fleischer. Biochem. Biophys. Res. Commun. 32, 512 (1968).PubMedCrossRefGoogle Scholar
  5. 5.
    Green, D. E., N. F. Haard, G. Lenaz and H. I. Silman. Proc. Nat. Acad. Sci. 60, 277 (1968).PubMedCrossRefGoogle Scholar
  6. 6.
    Green, D. E., H. D. Tisdale, R. S. Criddle and R. M. Bock. Biochem. Biophys. Res. Commun. 5, 81 (1961).PubMedCrossRefGoogle Scholar
  7. 7.
    Criddle, R. S., R. M. Bock, D. E. Green and H. Tisdale. Biochem. 1, 827 (1962).CrossRefGoogle Scholar
  8. 8.
    Richardson, S. H., H. O. Hultin and D. E. Green. Proc. Nat. Acad. Sci. 50, 821 (1963).PubMedCrossRefGoogle Scholar
  9. 9.
    Criddle, R. S. and L. Park. Biochem. Biophys. Res. Commun. 17, 74 (1964).PubMedCrossRefGoogle Scholar
  10. 10.
    Richardson, S. H., H. O. Hultin and S. Fleischer. Arch. Biochem. Biophys. 105, 254 (1964).PubMedCrossRefGoogle Scholar
  11. 11.
    Got, K. and J. B. Polya, Enzymologia 27, 63 (1964).PubMedGoogle Scholar
  12. 12.
    Woodward, D. O. and K. D. Munkres. Proc. Nat. Acad. Sci. 55, 872 (1966).PubMedCrossRefGoogle Scholar
  13. 13.
    Scheiderman, L. J. and I. G. Junga. Biochem. 7, 2281 (1968).CrossRefGoogle Scholar
  14. 14.
    Lenaz, G., N. F. Haard, A. Lauwers, D. W. Allmann and D. E. Green. Arch. Biochem. Biophys. 126, 746 (1968).PubMedCrossRefGoogle Scholar
  15. 15.
    Criddle, R. S. Ann. Rev. Plant Phys. 20, 239 (1969).CrossRefGoogle Scholar
  16. 16.
    Fleischer, S., H. Ozawa, W. L. Zahler and A. Saito. Unpublished observation.Google Scholar
  17. 17.
    Fleischer, S., W. L. Zahler and H. Ozawa. Biochem. Biophys. Res. Commun. 32, 1031 (1968).PubMedCrossRefGoogle Scholar
  18. 18.
    Ozawa, H., S. Fleischer and W. L. Zahler. In preparation.Google Scholar
  19. 19.
    We are grateful to Dr. A. Azzi and Dr. B. Chance for their assistance in obtaining low temperature spectra.Google Scholar
  20. 20.
    Fernandez-Moran, H., T. Oda, P. V. Blair and D. E. Green. J. Cell Biol. 22, 63 (1964).PubMedCrossRefGoogle Scholar
  21. 21.
    Racker, E. in Membranes of Mitochondria and Chloroplasts ( E. Racker, ed.) Van Nostrand Reinhold Co., New York 1970 p. 127.Google Scholar
  22. 22.
    Schatz, G. and J. Saltzgaber. Biochim. Biophys. Acta 180, 186 (1969).PubMedCrossRefGoogle Scholar
  23. 23.
    Senior, A. E. and D. H. MacLennan. J. Biol. Chem. 245, 5086 (1970).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1971

Authors and Affiliations

  • Sidney Fleischer
    • 1
  • Warren L. Zahler
    • 1
  • Hidehiro Ozawa
    • 2
  1. 1.Department of Molecular BiologyVanderbilt UniversityNashvilleUSA
  2. 2.Department of AnatomyNiigata UniversityNiigataJapan

Personalised recommendations