Biomembranes pp 257-288 | Cite as

The Isolation of the Surface Membranes of Animal Cells: A Survey

  • Leonard Warren
  • M. C. Glick


At present we are witnessing a rapidly growing interest in the cell surface structure. While some investigators are examining the essential nature of the membrane itself, others, such as virologists, developmental biologists, immunologists, and oncologists are studying the role of the surface membrane in processes relevant to their special interests. Although surface structure of the intact cell can be profitably studied in situ,invaluable information can often be obtained only after removal of the surface structure from the cells. It should be remembered, however, that the vigorous methods employed to remove the structure from the cell and to separate it from other cellular components may alter the membrane; i.e., there may be losses, additions by adsorptive processes, or enzymatic degradation of membrane components and even significant changes of protein configuration. Therefore, use of the isolated membrane should be approached with caution.


Sialic Acid Surface Membrane Brush Border Bile Canaliculus Ehrlich Ascites Tumor Cell 
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Copyright information

© Plenum Press, New York 1971

Authors and Affiliations

  • Leonard Warren
    • 1
  • M. C. Glick
    • 1
  1. 1.Department of Therapeutic Research School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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