The Use of GC-MS Techniques for the Analysis of Therapeutic Agents in Blood

  • Michael Lehrer
  • Arthur Karmen
Part of the Progress in Analytical Chemistry book series (PAC)


Combined Gas Chromatography-Mass Spectrometry has become an established method for detecting and quantifying compounds in complex mixtures (1, 2, 3). In the work described here, we studied various methods of applying this powerful technique to the quantitative assay of the anti-epileptic drug, diphenylhydantoin, in serum samples submitted to our clinical laboratory. Mass fragmentography, in which the elution of compounds from the GC column is monitored by monitoring for preselected specific ions or spectra of ions as a function of time was used with both electron impact (EI) and chemical ionization (CI) in order to assess the sensitivity and potential advantages of each technique.


Chemical Ionization Half Height Electron Impact Mode Chemical Ionization Mass Spectrum Chemical Ionization Mode 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    A.E. Gordon and A. Frigerio, J. Chrornatog., 73, 401 (1972).CrossRefGoogle Scholar
  2. 2.
    D.J. Jenden and A.K. Cho, Ann. Rev. Pharmacol., 13, 371, (1973).CrossRefGoogle Scholar
  3. 3.
    J.M. Strong and A.J. Atkinson Jr., Finnigan Spectra, 4, #3, Dec. 1974.Google Scholar
  4. 4.
    T. Chang and A.J. Glazko, J. Lab. Clin. Med., 75, 145 (1970).Google Scholar
  5. 5.
    M.A. Evenson, P. Jones, and B. Darcey, Clin.Chem., 16, 107 (1970).Google Scholar
  6. 6.
    E.M. Baylis, D.E. Fry, V. Marks, Clinica Chimica Acta, 30, 93 (1970).CrossRefGoogle Scholar
  7. 7.
    L. Palmer, L. Bertilsson, P. Collstei, and M. Rawlins, Clin. Pharm. Thera., 14, 827 (1973).Google Scholar
  8. 8.
    J. M. Strong and A.J. Atkinson, Jr., Anal. Chem., 44, 2287 (1972).CrossRefGoogle Scholar
  9. 9.
    R. Rane, M. Garle, O. Borga, and F. Sjoqvist, Clin. Pharm. Thera., 15, 39 (1974).Google Scholar
  10. 10.
    A. Frigerio, R. Fanelli, and B. Danieli, Chem. & Ind., 19, 769 (1972).Google Scholar
  11. 11.
    E.J. Bonelli, J.B. Knight, and M.S. Story, Finnigan Application Tips, #24 (1971).Google Scholar
  12. 12.
    Application Note #4, Varian Mat, (GmbH) Sept. 1971.Google Scholar
  13. 13.
    G.H. Draffan, R.A. Clare, and F.M. Williams, J. Chromatog., 75, 45 (1973).CrossRefGoogle Scholar
  14. 14.
    J. H. Goudie and D. Burnett, Clinica Chimica Acta, 43, 423 (1973).CrossRefGoogle Scholar
  15. 15.
    E. Brockmann-Hannsen and T. Oki, J. Pharm. Sci., 58, 370 (1969).CrossRefGoogle Scholar
  16. 16.
    H.J. Kupeferberg, Clinica Chimica Acta, 29, 283, (1970).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Michael Lehrer
    • 1
  • Arthur Karmen
    • 1
  1. 1.Department of Laboratory MedicineAlbert Einstein College of Medicine of Yeshiva UniversityBronxUSA

Personalised recommendations