The Use of GC-MS Techniques for the Analysis of Therapeutic Agents in Blood
Combined Gas Chromatography-Mass Spectrometry has become an established method for detecting and quantifying compounds in complex mixtures (1, 2, 3). In the work described here, we studied various methods of applying this powerful technique to the quantitative assay of the anti-epileptic drug, diphenylhydantoin, in serum samples submitted to our clinical laboratory. Mass fragmentography, in which the elution of compounds from the GC column is monitored by monitoring for preselected specific ions or spectra of ions as a function of time was used with both electron impact (EI) and chemical ionization (CI) in order to assess the sensitivity and potential advantages of each technique.
KeywordsChemical Ionization Half Height Electron Impact Mode Chemical Ionization Mass Spectrum Chemical Ionization Mode
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