In Vitro Radioisotopic Methods for Clinical Evaluation of Vitamin B12 and Folic Acid Metabolism

  • Sheldon P. Rothenberg
Part of the Progress in Analytical Chemistry book series (PAC)


Many important physiologic processes are mediated by substances present in concentrations too low to be measured with instrumentation dependent on colorimetric or fluorometric reaction systems. Consequently, the assay procedures for many hormones, enzymes, metabolic substrates and vitamins was dependent for many years on biological activity where sensitivity, if not precision, was greater. However, with the availability of radioactive isotopes which could be incorporated into these substances, a significant advance had been made because they could now be monitored at lower and more physiologic concentrations. Coupled with the availability of radioactive labels was the development of the competitive radioimmunoassay for insulin by Yalow and Berson (1) which probably was the singular most important advance in the field of biomedical measurement. This technique, first exploited for the measurement of insulin, has now been extended for the measurement of other hormones, both peptide and non-peptide, other types of proteins, vitamins and drugs, using, in addition to specific antibodies, other macromolecular binding ligands or receptor sites obtained from tissues or biological fluids.


Gastric Juice Binding Ligand Intrinsic Factor DEAE Cellulose Pernicious Anemia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Yalow, R.S. and Berson, S.A.: J. Clin. Invest. 39: 1157, 1960.CrossRefGoogle Scholar
  2. 2.
    Rothenberg, S. P.: J. Clin. Invest. 42: 1391, 1963.CrossRefGoogle Scholar
  3. 3.
    Rothenberg, S. P.: Metab., 22: 1075, 1973.CrossRefGoogle Scholar
  4. 4.
    Barakat, R. M. and Ekins, R. P.: Blood 21: 70, 1963.Google Scholar
  5. 5.
    Crossowicz, D., Sulitzeanu, D. and Merzbach, D.: Proc. Soc. Exp. Biol. Med., 109: 604, 1962.CrossRefGoogle Scholar
  6. 6.
    Frenkel, E. P., Keller, S. and McCall, M. S.: J. Lab. Clin. Med., 68: 510, 1966.Google Scholar
  7. 7.
    Carmel, R. and Coltman, C.A.: J. Lab. Clin. Med., 74: 967, 1969.Google Scholar
  8. 8.
    Rothenberg, S. P.: Blood 31: 44, 1968.Google Scholar
  9. 9.
    Lau, K. S., Gottlieb, C., Wasserman, L.R. and Herbert, V.: Blood 26: 202, 1965.Google Scholar
  10. 10.
    Hall, C.A. and Finkler, A.E.: Biochim. Biophys. Acta 78: 234, 1963.CrossRefGoogle Scholar
  11. 11.
    Hall, C.A. and Finkler, A. E.: J. Lab. Clin. Med., 65: 459, 1965.Google Scholar
  12. 12.
    Hall, C.A. and Finkler, A.E.: Biochim. Biophys. Acta 147: 186, 1967.CrossRefGoogle Scholar
  13. 13.
    Lawrence, C.: Blood 33: 899, 1969.Google Scholar
  14. 14.
    Bloomfield, F. J. and Scott, J. M..: Brit. J. Haemat., 22: 33, 1972.CrossRefGoogle Scholar
  15. 15.
    Allen, R.H. and Majerus, P.W.: J. Biol. Chem., 247: 7702, 1972.Google Scholar
  16. 16.
    Allen, R.H. and Majerus, P. W.: J. Biol. Chem.: 247: 7709, 1972.Google Scholar
  17. 17.
    Carmel, R. and Herbert, V.: Blood 40: 542, 1972.Google Scholar
  18. 18.
    Herbert, V., Bloomfield, J., Stebbins, R. and Scott, J.: J. Clin. Invest., 52: 39a, 1973.Google Scholar
  19. 19.
    Bloomfield, J., Scott, J., Herbert, V. and Stebbins, R.: Fed. Proc., 32: 892 (Abs.), 1973.Google Scholar
  20. 20.
    Taylor, K. B.: Lancet 2: 106, 1959.CrossRefGoogle Scholar
  21. 21.
    Schade, S. G., Abels, J. and Schilling, R. F.: J. Clin. Invest., 46: 615, 1967.CrossRefGoogle Scholar
  22. 22.
    Rothenberg, S. P., Kantha, K.R.K. and Ficarra, A.: J. Lab. Clin. Med., 77: 476, 1971.Google Scholar
  23. 23.
    Rothenberg, S. P.: Nature 200: 922, 1963.CrossRefGoogle Scholar
  24. 24.
    Ghitis, J.: Am. J. Clin. Nutr., 20: 1, 1967.Google Scholar
  25. 25.
    Kamen, B. A., and Gaston, J. D.: J. Lab. Clin. Med., (In Press).Google Scholar
  26. 26.
    Rothenberg, S. P. and da Costa, M.: J. Clin. Invest., 50: 719, 1971.CrossRefGoogle Scholar
  27. 27.
    Retief, F. P. and Huskisson, J.: J. Clin. Path., 23: 703, 1970.CrossRefGoogle Scholar
  28. 28.
    McCall, M. S., White, J. D. and Frenkel, E. P.: Proc. Soc. Exp. Biol. Med., 134: 536, 1970.CrossRefGoogle Scholar
  29. 29.
    Ricker, R. and Stollar, B. D.: Biochem., 6: 2001, 1967.Google Scholar
  30. 30.
    Rothenberg, S. P., Gizis, F. and Kamen, B.: J. Lab. Clin. Med., 74: 662, 1969.Google Scholar
  31. 31.
    Rothenberg, S. P.: Proceeding of International Atomic Energy Symposium, “In Vitro” Procedures with Radioisotopes in Clinical Medicine and Research. 1970.Google Scholar
  32. 32.
    Rothenberg, S. P.: Anal. Biochem., 16: 176, 1966.CrossRefGoogle Scholar
  33. 33.
    da Costa, M. and Rothenberg, S. P.: Brit. J. Haemat., 21: 121, 1971.CrossRefGoogle Scholar
  34. 34.
    Waxman, S., Schreiber, C. and Herbert, V.: Blood 38: 219, 1971.Google Scholar
  35. 35.
    Rothenberg, S. P., da Costa, M. and Rosenberg, Z.: New Eng. J. Med., 286: 1335, 1972.CrossRefGoogle Scholar
  36. 36.
    da Costa, M. and Rothenberg, S. P.: J. Lab. Clin. Med., (In Press).Google Scholar
  37. 37.
    Waxman, S. and Schreiber, C.: Blood 42: 281, 1973.Google Scholar
  38. 38.
    Rothenberg, S. P., da Costa, M., and Lawson, J.: Blood, (In Press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1974

Authors and Affiliations

  • Sheldon P. Rothenberg
    • 1
  1. 1.New York Medical CollegeNew YorkUSA

Personalised recommendations