Applications of Mass Spectrometry in Drug Metabolism and Related Fields

  • W. J. A. Vanden
Part of the Progress in Analytical Chemistry book series (PAC, volume 176)


The role of mass spectrometry (MS) in drug metabolism studies is to supply qualitative and possibly quantitative information from micrograms of partially purified isolates. The earlier in an isolation procedure the great sesitivity and structure elucidating power of MS can be focused upon a sample from a small quantity of biological material the greater the value of the method. Manipulation of grams of tissues or milliliters of fluid is preferred to handling kilogram or liter quantities. The ability to identify metabolites in a moderately complex matrix from small amounts of tissue, urine, etc., facilitates work with individual animals and offers the possibility of carrying out dynamic experiments (e.g., following changes in metabolite pattern with time). Direct probe (DP)-MS is useful in such studies but often it is combined gas-liquid chromatography (GLC)-MS, independently or together with DP-MS, which is especially effective in dealing with mixtures of this type.


Drug Metabolism Direct Probe Mass Spectrometric Technique Reactive Functional Group Probe Tube 
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Copyright information

© Plenum Press, New York 1973

Authors and Affiliations

  • W. J. A. Vanden
    • 1
  1. 1.Merck Sharp & Dohme Research LaboratoriesRahwayUSA

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