Host Immunoreactivity to CSA, a Tissue-Specific Antigen of Normal and Neoplastic Human Intestine
The GW-39 tumor system consists of a human signet-ring cell carcinoma of the human colon which is serially transplantable in normal, unconditioned, adult golden hamsters either in the cheek pouch or intramuscularly (5). The microscopic morphology of GW-39, after staining with PAS, indicates that intracytoplasmic mucin is being produced by the tumor. Signet-ring cells are also prevelant. This tumor system has now been maintained by us in hamsters for about 10 yr, retaining human species-specific and organ-specific properties. For example, the few cells amenable to karyotyping have revealed a human pseudodiploid karyotype. As we reported earlier (3), one of the more interesting properties of GW-39 cells, which is consistent with their human origin, is their capacity to synthesize the carcinoembryonic antigen (CEA) of Gold and Freedman (2). Since this human colonic carcinoma retained a tumor-associated glycoprotein such as CEA even after long-term propagation in animal hosts, we reasoned that other human and perhaps organ-associated substances could likewise be detectable in this tumor model. The purpose of this paper is to describe the extraction and identification of an antigen, CSA, present in normal and neoplastic digestive tract tissue.
KeywordsFiltration Phenol Ster
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