Biochemical Characteristics of 8-Azaguanine Resistant Human Lymphoblast Mutants Selected in Vitro

  • G. Nuki
  • J. Lever
  • J. Edwin Seegmiller
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 41A)


Much of recent progress in our understanding of the regulation of purine metabolism in man has resulted from in vitro studies on cells from patients with inborn errors of metabolism (1, 2).


Minimal Inhibitory Concen Purine Metabolism Human Lymphoblast Human Embryonic Lung Fibroblast PRPP Synthetase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Seegmiller, J. E., Rosenbloom, F. M., and Kelley, W. N., Science 155, 1682 (1967).PubMedCrossRefGoogle Scholar
  2. 2.
    Kelley, W. N., Purine and pyrimidine metabolism of cells in culture. In Growth, Nutrition and Metabolism of Cells in Culture, Eds. G. H. Rothblat and V. J. Cristofolo. Academic Press, New York, 1972.Google Scholar
  3. 3.
    Gots, J. S. Regulation of purine and pyrimidine metabolism. In Metabolic Pathways, 3rd Ed., Vol. 5, Metabolic Regulation, Ed. H. J. Vogel, New York, Academic Press, 1971, p. 225.Google Scholar
  4. 4.
    Kelley, W. N., and Meade, J. C., J. Biol. Chem. 246, 2953 (1971).PubMedGoogle Scholar
  5. 5.
    Kirkman, H. N., Enzyme Defects. In Progress in Medical Genetics, Vol. VIII, Eds. A. G. Steinberg and A. G. Bearn, Grune and Stratton, 1972.Google Scholar
  6. 6.
    Liebeman, I., and Ove, P., Proc. Nat. Acad. Sci. USA 45 867 (1959).CrossRefGoogle Scholar
  7. 7.
    Littlefield, J. W., Proc. Nat. Acad. Sci. USA 50, 568 (1963).CrossRefGoogle Scholar
  8. 8.
    Chu, E. H. Y., Brimer, P., Jacobson, K. B., and Merriam, E. V., Genetics 62, 359 (1969).PubMedGoogle Scholar
  9. 9.
    Gillin, F. D., Roufa, D. J., Beaudet, A. L., and Caskey, C. T., Genetics 72, 239 (1972).PubMedGoogle Scholar
  10. 10.
    Albertini, R. J., and DeMars, R., Science 169, 482 (1970).CrossRefGoogle Scholar
  11. 11.
    DeMars, R., and Held, K. R., Humangenetik 16, 87 (1972).PubMedCrossRefGoogle Scholar
  12. 12.
    Sato, K., Slesinski, R. S., and Littlefield, J. W., Proc. Nat. Acad. Sci. USA 69, 1244 (1972).CrossRefGoogle Scholar
  13. 13.
    Levy, J. A., Virolainen, M., and Defendi, V., Cancer 22, 517 (1968).PubMedCrossRefGoogle Scholar
  14. 14.
    Levy, J. A., Buell, D. N., Creech, C., Hirshaut, Y., and Silverberg, H., J. Nat. Cancer Inst. 46, 647 (1971).PubMedGoogle Scholar
  15. 15.
    Wood, A. W., Becker, M. A., and Seegmiller, J. E., Biochem. Genet., In Press.Google Scholar
  16. 16.
    Coffino, P., Laskow, R., and Scharff, M. D., Science 167 186 (1970).PubMedCrossRefGoogle Scholar
  17. 17.
    Lerner, R. A., McConaney, P. J., and Dixon, F. J., Science 173, 60 (1971).PubMedCrossRefGoogle Scholar
  18. 18.
    Fujimoto, W. Y., and Seegmiller, J. E., Proc. Nat. Acad. Sci. USA 65, 577 (1970).CrossRefGoogle Scholar
  19. 19.
    Watson, B., Gormley, I. P., Gardiner, S. E., Evans, H. J., and Harris, H. Exp. Cell Res. 75, 401 (1972).PubMedCrossRefGoogle Scholar
  20. 20.
    Kelley, W. N., Greene, M. L., Rosenbloom, F. M., Henderson, J. F., and Seegmiller, J. E., Ann. Intern. Med. 70, 155 (1969).PubMedGoogle Scholar
  21. 21.
    Harris, M., J. Cell Physiol. 78, 177 (1971).PubMedCrossRefGoogle Scholar
  22. 22.
    Mezger-Freed, L., Nature (New Biol.) 235, 245 (1972).CrossRefGoogle Scholar
  23. 23.
    Riccardi, V. M., and Littlefield, J. W., Exp. Cell Research 74, 417 (1972).CrossRefGoogle Scholar
  24. 24.
    Raivio, K. O., and Seegmiller, J. E., Biochim. Biophys. Acta 299, 273 (1972).Google Scholar
  25. 25.
    Greene, M. L., Boyle, J. A., and Seegmiller, J. E., Science 167, 887 (1970).PubMedCrossRefGoogle Scholar
  26. 26.
    Brenton, D., Nuki, G., Astrin, K., Lever, J., Cruikshank, M., and Seegmiller, J. E., In Preparation.Google Scholar
  27. 27.
    Rosenbloom, F. M., Henderson, J. F., Caldwell, I. C., Kelley, W. N., and Seegmiller, J. E., J. Biol. Chem. 243, 1166 (1968).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • G. Nuki
    • 1
  • J. Lever
    • 1
  • J. Edwin Seegmiller
    • 1
  1. 1.Department of MedicineUniversity of California San DiegoLaJollaUSA

Personalised recommendations