Purification and Characterization of a Neural Hypo-Xanthine-Guanine-Phosphoribosyltransferase (HGPRT)

  • Wolf Gutensohn
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 41A)


Starting from investigations on RNA-metabolism in rat brain and because of the close connection of HGPRT with a neurological disorder in man we became interested in the properties of a neural HGPRT in the laboratory of Gordon Guroff at the National Institute of Health. We adjusted the known HGPRT-assay using DOWEX 1 × 8 for separation of the product to smaller scale and used this assay (W.Gutensohn,G.Guroff, Analytical Biochemistry 47,132(1972)) in all the following experiments.


Ammonium Sulfate Ammonium Sulfate Fraction Subunit Molecular Weight Crude Supernatant Soluble Supernatant 
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Wolf Gutensohn
    • 1
  1. 1.Max-Planck-Institut für BiochemieMartinsried, MunichGermany

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