Purification and Characterization of a Neural Hypo-Xanthine-Guanine-Phosphoribosyltransferase (HGPRT)
Starting from investigations on RNA-metabolism in rat brain and because of the close connection of HGPRT with a neurological disorder in man we became interested in the properties of a neural HGPRT in the laboratory of Gordon Guroff at the National Institute of Health. We adjusted the known HGPRT-assay using DOWEX 1 × 8 for separation of the product to smaller scale and used this assay (W.Gutensohn,G.Guroff, Analytical Biochemistry 47,132(1972)) in all the following experiments.
KeywordsAmmonium Sulfate Ammonium Sulfate Fraction Subunit Molecular Weight Crude Supernatant Soluble Supernatant
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