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Incorporation of 3H-Hypoxanthine in PHA Stimulated HG-PRT Deficient Lymphocytes

  • C. H. M. M. de Bruyn
  • T. L. Oei
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 41A)

Abstract

Autoradiography with labeled substrates on cultured fibroblasts has been used to visualize HG-PRT deficiency: cells of deficient individuals lack the ability to incorporate hypoxanthine or guanine into nucleic acids, because these purine bases can not be converted to their corresponding mononucleotides (1,2). The alternative pathway to form IMP or GMP via inosine or guanosine is not likely, for, although nucleoside phosphorylase is present, there is no definite evidence for the existence of inosine- or guanosine kinase in human cells (3,4).

Keywords

Purine Metabolism Purine Base Label Substrate Deficient Individual Heavy Label 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • C. H. M. M. de Bruyn
    • 1
  • T. L. Oei
    • 1
  1. 1.Department of Human Genetics, Faculty of MedicineUniversity of NijmegenThe Netherlands

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