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Purine Nucleoside Metabolism in Escherichia Coli

  • Bjarne Jochimsen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 41A)

Abstract

This manuscript will mainly deal with purine nucleoside metabolism, and I would like to stress that the organism studied is the enteric bacterium Escherichia coli with its natural habitat:the human rectum.

Keywords

Adenosine Deaminase Enteric Bacterium Purine Nucleoside Purine Nucleoside Phosphorylase Adenylosuccinate Lyase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Assays, purine nucleoside kinase assays were made by mixing equal volumes of fraction and reaction mixture: (0,14 M Tris-Cl s 7.8 (6.5),0,o7 M MgC1, 0,07 M KC1, 2mM ATP and 1mM C-(U)-nucleoside (50.0000 cpm/l). Reaction mixture plus enzyme was incubated at 37 C for 30) minutes and 15/ul samples were taken and spotted on poly-ethylene-ifiine coated plastic sheets. Plates were developed with water, and start spots containing nucleotides were cut out, and counted.Google Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Bjarne Jochimsen
    • 1
  1. 1.Institute of Biological Chemistry BUniversity of CopenhagenDenmark

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