Abstract
This manuscript will mainly deal with purine nucleoside metabolism, and I would like to stress that the organism studied is the enteric bacterium Escherichia coli with its natural habitat:the human rectum.
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Gots,J.S. J.Bacterio1.112(1972)910–916
Hammer-Jespersen,K. Eur.J.Biochem.l9(1971)533–538
Hochstadt-Ozer,J. J.Biol.Chem.246(1971)5294–5303
Hoffmeyer,J. J.Bacterio1.106(1971)14–24
Karlström,0. J.Bacterio1.95(1968)1069–1077
Krenitsky,T.A. J.Biol.Chem.245(1970)2605–2611
Munch-Petersen,A. Eur.J.Biochem.6(1968)432–442
Nygaard,P. Personal communication
Remy,C.N. J.Bacteriol.96(1968)76–85
Assays, purine nucleoside kinase assays were made by mixing equal volumes of fraction and reaction mixture: (0,14 M Tris-Cl s 7.8 (6.5),0,o7 M MgC1, 0,07 M KC1, 2mM ATP and 1mM C-(U)-nucleoside (50.0000 cpm/l). Reaction mixture plus enzyme was incubated at 37 C for 30) minutes and 15/ul samples were taken and spotted on poly-ethylene-ifiine coated plastic sheets. Plates were developed with water, and start spots containing nucleotides were cut out, and counted.
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© 1974 Plenum Press, New York
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Jochimsen, B. (1974). Purine Nucleoside Metabolism in Escherichia Coli. In: Sperling, O., De Vries, A., Wyngaarden, J.B. (eds) Purine Metabolism in Man. Advances in Experimental Medicine and Biology, vol 41A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3294-7_17
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DOI: https://doi.org/10.1007/978-1-4684-3294-7_17
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