Incorporation of Purine Analogs into the Nucleotide Pools of Human Erythrocytes

  • R. E. ParksJr.
  • Phyllis R. Brown
  • Chong M. Kong
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 41A)


For the past decade this laboratory has devoted much of its attention to an examination of various facets of purine metabolism in human erythrocytes. These cells do not have the complete pathway for the de novo synthesis of purines and do not make nucleic acids. On the other hand, they have an active nucleotide metabolism and contain the salvage enzymes, hypoxanthine-guanine phosphoribosyl transferase (HGPRTase), adenine phosphoribosyl transferase (APRTase) and adenosine kinase. In view of the fact that the activities of certain enzymes of purine metabolism are quite high (e.g., purine nucleoside phosphorylase occurs at a level of about 15 umolar units/ml of erythrocytes) and the total mass of erythrocytes in the adult human being is in excess of two liters, it appears that these cells play an important and perhaps not yet fully appreciated role in the whole body economy of purines in man. Therefore, we believe that the human erythrocyte provides a very useful model system for the examination of purine metabolism in man as well as for investigations of the action of certain purine and purine nucleoside antimetabolites, many of which are important in medicine.


High Pressure Liquid Chromatography Human Erythrocyte Adenosine Deaminase Purine Nucleoside Purine Metabolism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Agarwal, K. C. and Parks, Jr., R. E. (1972), Mol. Pharmacol. 8, 128PubMedGoogle Scholar
  2. Agarwal, R. P. Sagar, S. M. and Parks, Jr., R. E. (1973), Fed. Proc. 32, 512Google Scholar
  3. Agarwal, R. P., Scholar, E. M., Agarwal, K. C. and Parks, Jr., R. E. (1971), Biochem. Pharmacol. 20, 1341CrossRefGoogle Scholar
  4. Brown, P. R. (1970), J. Chromatog. 52, 257CrossRefGoogle Scholar
  5. Brown, P. R. (1973), “High Pressure Liquid Chromatography”, Academic Press, N. Y.Google Scholar
  6. Brown, P. R., Agarwal, R. P., Gell, J. and Parks, Jr., R. E. (1972), Comp. Biochem. Physiol. 433, 891Google Scholar
  7. Brown, P. R. and Parks, Jr., R. E. (1973), this volume Dixon, M. (1952), Biochem. J. 55, 161Google Scholar
  8. Goldberg, N. D., Dahl, J. L. and Parks, Jr., R. E. (1963), J. Biol. Chem. 238, 3109PubMedGoogle Scholar
  9. Horvath, C., Preiss, B. and Lipsky, S. (1967), Anal. Chem. 39, 1422PubMedCrossRefGoogle Scholar
  10. Krenitsky, T. A., Papaioannou, R. and Elion, G. B. (1969), J. Biol. Chem. 244, 1263PubMedGoogle Scholar
  11. Manohar, S. V., Lerner, M. H. and Rubenstein, D. (1968), Can. J. Biochem. 46, 455Google Scholar
  12. Meyskens, F. L. and Williams, H. E. (1971), Biochim. Biophys. Acta 240, 170Google Scholar
  13. Miller, R. L. and Bieber, A. L. (1968), Biochemistry 7, 1420PubMedCrossRefGoogle Scholar
  14. Miller, R. L. and Bieber, A. L. (1969), Biochemistry 8, 603PubMedCrossRefGoogle Scholar
  15. Moore, E. C. and LePage, G. A. (1958), Cancer Res. 18, 1075PubMedGoogle Scholar
  16. Nelson, D. J., Bugge, C. J. L., Krasny, H. C. and Zimmerman, T. P. (1973), J. Chromatog. 77, 181CrossRefGoogle Scholar
  17. Parks, Jr., R. E. and Agarwal, R. Vol. 7, 3rd ed. (Paul D. Boyer, Parks, Jr., R. E. and Brown, P. R. press)Google Scholar
  18. Parks, Jr., R. E., Brown, P. R., Cheng, Y-C., Agarwal, K. C., Kong, C. M., Agarwal, R. P. and Parks, Christopher C. (1973), Comp. Biochem. Physiol. 45B, 355Google Scholar
  19. Ross, A. F., Agarwal, K. C., Chu, S.-H. and Parks, Jr., R. E. (1973), Biochem. Pharmacol. 22, 141PubMedCrossRefGoogle Scholar
  20. Scholar, E. M., Brown, P. R., Parks, Jr., R. E. and Calabresi, P. (1973), Blood (in press )Google Scholar
  21. Way, J. L. and Parks, Jr., R. E. (1958), J. Biol. Chem. 231, 467PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • R. E. ParksJr.
    • 1
  • Phyllis R. Brown
    • 1
  • Chong M. Kong
    • 1
  1. 1.Division of Biological and Medical SciencesBrown UniversityProvidenceUSA

Personalised recommendations