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Human Phosphoribosylpyrophosphate (PP-ribose-P) Synthetase: Properties and Regulation

  • I. H. Fox
  • W. N. Kelley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 41A)

Abstract

PP-ribose-P and the enzyme catalyzing its synthesis, PP-riboseP synthetase, were initially discovered by Kornberg, Lieberman and Simms in 1955. PP-ribose-P was subsequently recognized to be an essential substrate for purine, pyridine and pyrimidine biosynthesis, as well as for the formation of other compounds. Recent studies on the control of PP-ribose-P synthesis in bacteria, Ehrlich tumor ascites cells and human erythrocytes have suggested a complicated system of control depending upon ribose-5-P availability, cell energy levels and feedback inhibition from end products. Additional observations have suggested that the intracellular concentration of PP-ribose-P has a critical regulatory role in the biosynthesis of purines de novo in man. In order to clarify the regulation of PP-ribose-P synthesis, we have purified and studied this enzyme from human erythrocytes.

Keywords

Human Erythrocyte Apparent Molecular Weight Kinetic Mechanism Sedimentation Coefficient Ehrlich Tumor Ascites Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Atkinson, D. E. and Fall, L. 1967. Adenine triphosphate conservation in biosynthetic regulation: Escherichia coli phosphoribosylpyrophosphate synthetase. J. Biol. Chem. 242: 3241–3242.PubMedGoogle Scholar
  2. Fox, I. H. and Kelley, W. N. 1971. Human phosphoribosylpyrophosphate synthetase: Distribution, purification and properties. J. Biol. Chem. 246: 5739–5745.PubMedGoogle Scholar
  3. Fox, I. H. and Kelley, W. N. 1972. Human phosphoribosylpyrophosphate synthetase: Kinetic mechanisms and end-product inhibition. J. Biol. Chem. 247: 2126–2131.PubMedGoogle Scholar
  4. Kornberg, A., Lieberman, I. and Simms, E. S. 1955. Enzymatic synthesis and properties of 5-phsphoribosylpyrophosphate. J. Biol. Chem. 215: 389.PubMedGoogle Scholar
  5. Switzer, R. L.: 1967. End product inhibition of phosphoribosylpyrophosphate synthetase. (Abstract). Fed. Proc. 26: 560.Google Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • I. H. Fox
    • 1
    • 2
  • W. N. Kelley
    • 1
    • 2
  1. 1.University of TorontoCanadaUSA
  2. 2.Duke University Medical CenterDurhamUSA

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