Development of an In-Vitro Method for Simulation of Oxygen Transport in the Microcirculation
Measurement of oxygen transport rates in the microcirculation has been the subject of numerous in vivo investigations and considerable progress has been made. However, there are experimental difficulties in exact determination of several important parameters including the capillary wall boundary conditions and the capillary dimensions. These difficulties have constituted the incentive for our continuing development of an in vitro system in which the capillary dimensions are determined precisely by light and electron microscopy; the flow rate is carefully regulated; the inlet concentration of red cells or hemoglobin is controlled independently; the fractional saturation of hemoglobin is measured spectrophotometrically; and the boundary conditions in the silicone rubber capillary bed can be computed by established mathematical techniques. Accurate measurement of these variables should prove to be useful in testing and validating the several mathematical models which have been used in simulation of microcirculatory oxygen transport. A preliminary report on the project has described the basic features of the method (Boland, et al, 1984).
KeywordsOxygen Saturation Nusselt Number Silicone Rubber Oxygen Transport Capillary Wall
Unable to display preview. Download preview PDF.
- Anon., 1968, Encyclopedia of Polymer Science and Technology8: 630, J. Wiley and Sons.Google Scholar
- Baxley, P. T., and Heliums, J. D., 1984, Simulation of oxygen transport in the microcirculation, Ann. Biomed. Eng., in press.Google Scholar
- Boland, E. J., Unno, H., Olson, J. S., and Heliums, J. D., 1984, An in-vitro method for simulation of oxygen transport in the microcirculation, Proceedings of International Society on Oxygen Transport to Tissues Meeting, Plenum Publishing Corp., in press.Google Scholar
- Coin, J. T., and Olson, J. S., 1979, The rate of oxygen uptake by human red blood cells, J. Bio. Chem., 254, 1178–1190.Google Scholar
- Pittman, R. N., and Duling, B. R., 1975a, A new method for the measurement of percent oxyhemoglobin, J. Appl. Physiol.38: 315–320.Google Scholar
- Pittman, R. N., and Duling, B. R., 1975b, Measurement of percent oxyhemoglobin in the microvasculature, J. Appl. Physiol.38: 321–327.Google Scholar
- Sinha, A. K., 1969, O2 uptake and release by red cells through capillary wall and plasma layer, Ph.D. Thesis in Physiology, University of California, San Francisco, CA.Google Scholar
- Yap, E., 1984, Mathematical Modeling of oxygen transport in the microcirculation, M.S. Thesis in Chemical Engineering, Rice University.Google Scholar