Quantitative Analysis of Reflection Spectra: Evaluation of Simulated Reflection Spectra

  • J. Hoffmann
  • D. W. Lübbers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 191)


Reflection photometry is a useful tool to study in vivo kinetic changes of hemoglobin, myoglobin, and the cytochromes. The problem of quantifying reflection spectra is still not satisfactorily solved. To compare the validity and the limits of different methods to analyze optical spectra (dual wavelength evaluation, reference spectra method, two-flux evaluation method of multicomponent systems), calculations were performed on simulated reflection spectra. The simulation of the reflection spectra was carried out, using the two-flux theory of Kubelka and Munk (1931), (Kubelka 1948, 1954).


Reflection Spectrum Optical Signal Characteristic Wavelength Scattering Coefficient Isobestic Point 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • J. Hoffmann
    • 1
  • D. W. Lübbers
    • 1
  1. 1.Max-Planck-Institut für SystemhysiologieRheinlanddamm 201Dortmund 1Germany

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