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The Near Infrared (Nir) Absorption Band of Cytochrome aa3 in Purified Enzyme, Isolated Mitochondria and in The Intact Brain in Situ

  • H. H. Keizer
  • F. F. Jöbsis-Vander Vliet
  • S. S. Lucas
  • C. A. Piantadosi
  • A. L. Sylvia
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 191)

Abstract

The potential value of monitoring the redox state of cytochrome c oxidase (cytochrome aa 3 or cyt aa 3) as an index to cerebral energetics was highlighted in the preceding contribution (Sylvia et al., 1985), Hypoxically induced changes in the redox state of cyt aa 3 correlated well with creatine phosphate fluctuations. That study was performed using the well described absorption peak of reduced heme a. in the visible part of the spectrum as the marker (Jöbsis et al., 1977). Even as successful as that effort has proved to be, a considerable drawback exists in the fact that, except for very thin skulled animals such as rats and birds, the cerebral cortex must be exposed to obtain the optical signals. In order to be maximally useful, a totally non-invasive approach should be provided for observations on larger species including humans. Not only would this provide a greater range of applicability, including routine monitoring of patients, but the importance of preserving normal intra-cranial pressure relations can hardly be overstressed.

Keywords

Cerebral Oxygen Lorentzian Line Shape Isolate Mitochondrion Infrared Absorption Band Purify Enzyme Preparation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • H. H. Keizer
    • 1
  • F. F. Jöbsis-Vander Vliet
    • 1
  • S. S. Lucas
    • 1
  • C. A. Piantadosi
    • 1
  • A. L. Sylvia
    • 1
  1. 1.Departments of Physiology and MedicineDuke University Medical CenterDurhamUSA

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