The Near Infrared (Nir) Absorption Band of Cytochrome aa3 in Purified Enzyme, Isolated Mitochondria and in The Intact Brain in Situ
The potential value of monitoring the redox state of cytochrome c oxidase (cytochrome aa 3 or cyt aa 3) as an index to cerebral energetics was highlighted in the preceding contribution (Sylvia et al., 1985), Hypoxically induced changes in the redox state of cyt aa 3 correlated well with creatine phosphate fluctuations. That study was performed using the well described absorption peak of reduced heme a. in the visible part of the spectrum as the marker (Jöbsis et al., 1977). Even as successful as that effort has proved to be, a considerable drawback exists in the fact that, except for very thin skulled animals such as rats and birds, the cerebral cortex must be exposed to obtain the optical signals. In order to be maximally useful, a totally non-invasive approach should be provided for observations on larger species including humans. Not only would this provide a greater range of applicability, including routine monitoring of patients, but the importance of preserving normal intra-cranial pressure relations can hardly be overstressed.
KeywordsCerebral Oxygen Lorentzian Line Shape Isolate Mitochondrion Infrared Absorption Band Purify Enzyme Preparation
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- Jöbsis, F.F., Keizer, J.H., LaManna, J.C. and Rosenthal, M., 1977, Reflectance spectrophotometry of cytochrome a,a 3 in vivo J. Appl. Physiol.: Respirat. Environ. Exercise Physiol. 43: 858–872.Google Scholar
- Jöbsis-VanderVliet, F.F., 1985, Lorentzian analysis of near infrared absorption band of native cytochrome c oxidase, in preparation.Google Scholar
- Jöbsis-Vander Vliet, F.F., Piantadosi, C.A., Sylvia, A.L., Lucas, S.K. and Keizer, H.H., 1985, Near infra red monitoring of cerebral oxygen sufficienty, 1. Spectra of sytochrome c oxidase, Neurol. Res., in press.Google Scholar
- Sylvia, A.L., Piantadosi, C.A. and Jobsis-VanderVliet, F.F., 1985, Cerebral bioenergetics and in vivo cytochrome c oxidase redox relationships, Adv. Exp. Med. Biol, in press.Google Scholar