Membrane Potential and Ca Influx in Hypoxic and Normoxic Carotid Body Type-I Cells
The carotid body is described as being a chemoreceptor since 1928 (de Castro). Under hypoxia, the natural stimulus, transmitter like dopamine, and Ach are released (Fidone et al., 1982; Eyzaguirre et al., 1965) and chemoreceptor discharge is increased (Delpiano and Acker, 1980). Till now it is not clear, whether this chemoreceptive process is located in the glomus cell membrane or in the adjacent nerve endings. Working with cells in primary tissue culture we can investigate the stimulation process on isolated carotid body cells. As previous studies showed (Pietruschka and Schäfer, 1976) fine structure of these cells, especially the dense cored vesicles, is preserved in culture.
KeywordsGlioma Cell Calcium Influx Carotid Body Dense Core Vesicle Glomus Cell
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- Chen, I., and Yates, R.D., 1984, Two types of glomus cells in the rat carotid body as revealed by α-bungarotoxin binding, J. Neuropathol., 13:281–302.Google Scholar
- De Castro, F., 1928, Sur la structure et l’innervation du sinus carotidien de l’homme et des mammifères. Nouveaux faits sur l’innervation et la fonction du glomus caroticum, Trav. Lab. Rech. Biol. Univ. Madrid, 25:330–380.Google Scholar
- Eyzaguirre, C., Baron, M., and Gallego, R., 1977, Intracellular studies of carotid body cells: effects of temperature, “natural” stimuli and chemical substances, in: 2 “Tissue Hypoxia and Ischemia”, Adv. Exp. Med. Biol., Vol. 78, M. Reivich, R. Coburn, S. Lahiri, and B. Chance (eds.), Plenum Press, New York-London, pp.209–223.Google Scholar
- Eyzaguirre, C., Koyano, H., and Taylor, J.R., 1965, Presence of acetylcholine and transmitter release from carotid body chemoreceptors, J. Physiol. (Lond.), 178:463–476.Google Scholar
- Pietruschka, F., and Schäfer, D., 1976, Fine structure of chemosen-sitive cells (glomus caroticum) in tissue culture, Cell Tiss. Res., 168:55–63.Google Scholar