In Vivo Arterial Platelet-Vessel Wall Interaction and Thrombosis: Induction, On-Line Registration and Ultrastructural Control
A technique for induction and on-line quantification of local platelet thrombi in mesenteric arteries of small laboratory animals was developed and standardized in our laboratory.
In the past, this model was used to study the nature of platelet-vessel wall interaction in the living animal. The ultrastructure of the experimental intimai lesion and the vessel wall regeneration were assessed by transmission electron microscopy (TEM), both in normal and pathologic conditions.
Scanning electron microscopy (SEM) now shows the ultramorpho-logy of platelet thrombi on the experimentally injured arterial segment following topical superfusion with ADP, mepacrine or platelet-activating factor (PAF). The application of these substances, each with proper bio-activity, leads to distinct types of platelet thrombi. Mepacrine or PAF superfusion causes large thrombotic masses, as compared to control, ADP induced thrombi, and seems toxic for the endothelial cells. Mepacrine thrombi differ significantly from PAF thrombi in their platelet density, degree of platelet activation and in their relation to the endothelium that surrounds the experimental lesion. Furthermore, PAF superfusion induces a phenomenon of spontaneous regeneration of the thrombus after its forced embolization. This is probably due to some unknown bio-action of PAF in the vessel wall.
KeywordsMesenteric Artery Small Laboratory Animal Platelet Thrombus Platelet Mass Thrombotic Phenomenon
Unable to display preview. Download preview PDF.
- Bourgain, R.H., 1978b, Inhibition of PGI2 (prostacyclin) synthesis in the arterial wall enhances the formation of white platelet thrombi in vivo, Haemostasis, 7:252–255.Google Scholar
- Bourgain, R.H., Andries, R. and Finne, E., 1979, The role of prostaglandins in platelet-vessel wall interactions, Thér., 239:161–163.Google Scholar
- Bourgain, R.H., Andries, R., Biagi, G. and Finne, E., 1981, The effect of arachidonic acid on platelet-vessel interaction, Arch.int.Phar-macodyn.Thér., 250:302–304.Google Scholar
- Bourgain, R.H., Vermarien, H., Andries, R., Vereecke, F., Jacqueloot, J., Rennies, J., Blockeel, E. and Six, F., 1984(in press), A standardized “in vivo” model for the study of experimental arterial thrombosis. Description of a method., Proc.Int.Symp. “Oxygen Trans-port to Tissue”, U.S.A. 1983.Google Scholar
- Hladovec, J., 1971, Experimental arterial thrombosis in rats with continuous registration, Thrombos.Diathes.Haemorrh., 26:407–410.Google Scholar
- Maes, L., Andries, R. and Bourgain, R.H., 1984a (in press), Endothelial injury and platelet thrombosis in mesenteric arteries of rats. A scanning electron microscopic study., Microvasc.Res. Google Scholar
- Maes, L., Bourgain, R.H., Andries, R. and Warson, F., 1984b (in press), the effect of PAF-acether on in vivo arterial platelet-vessel wall interaction in the guinea pig, Atherosclerosis. Google Scholar
- Potvliege, P.R. and Bourgain, R.H., 1979, Inhibition by suloctidil of the early wall reaction induved by local thrombosis, Eur.J. Rheum.Inflamm., 2:204–207.Google Scholar
- Potvliege, P.R., Maes, L., Warson, F. and Bourgain, R.H., 1984(in press), The effect of the inhibition of platelet function on the development of the primary atherogenic lesion in rats on a fat-cholesterol rich diet, Br.J.exp.Pathol. Google Scholar
- Ubatuba, F., Moncada, S. and Vane, J.R.,1979, The effect of prostacyclin on platelet behaviour, thrombus formation in vivo and bleeding time, Thromb.Haemostas.(Stuttg.), 41:425–435.Google Scholar
- Vargaftig, B.B., Chignard, M., Le Couedic, J.P. and Benveniste, J., 1980, One, two, three or more pathways for platelet aggregation, Acta Med.Scand.(Suppl.), 642:23–29.Google Scholar
- Weichert, W., Paulins, V. and Breddin, H.K., 1983, Laser induced thrombi in rat mesenteric vessels and antithrombotic drugs, Haemo-stasis, 13:61–71.Google Scholar