Advertisement

Oxygen Permeability of Methemoglobin Solutions Soaked in Millipore Filters

  • L. Hoofd
  • A. Lamboo
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 191)

Summary

For the measurement of gas diffusion through liquids, Millipore filters are an interesting and easy tool. They keep the fluid in place, and layer thickness can be determined precisely and easily. We soaked Millipore type SM filters with solutions containing 1.66 mmol/1 methemoglobin to measure the oxygen permeability at 25° C, and compared the results with former measurements in liquid layers. Data scattered between 1.0 and 1.5 10-11 mol • m-1 • kPa-1 • sec-1 and a decrease with increasing KCl concentration, as found for liquid layers, could neither be confirmed nor rejected. Nitrogen/oxygen permeability ratio of 0.465 ± 0.016 SE was in agreement with literature data.

Keywords

Oxygen Partial Pressure Liquid Layer Oxygen Electrode Millipore Filter Oxygen Permeability 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Breepoel, P. M., de Koning, J., and Hoofd, L., 1982, Diffusion of oxygen in methemoglobin solutions. Dependence on salt concentration. Biochem. Biophys. Res. Commun., 109: 848–850.PubMedCrossRefGoogle Scholar
  2. Cadman, A. D., Fleming, R., and Guy, R. H., 1981, Diffusion coefficient determination using a filter-paper diaphragm cell technique. J. Pharm. Pharmacol., 33: 121–123.PubMedCrossRefGoogle Scholar
  3. Hoofd, L., de Koning, J., Lamboo, A., and Kreuzer, F., Determination of permeabilities for two gases from recording the partial pressure of one gas, in preparation.Google Scholar
  4. Kimmich, H. P., and Kreuzer, F., 1969, Telemetry of respiratory oxygen pressure in man at rest and during exercise, in: “Oxygen Pressure Recording in Gases, Fluids, and Tissues”, Progr. Resp. Res., Vol. 3, F. Kreuzer, ed., Karger, Basel, pp 22–41.Google Scholar
  5. Scholander, P. F., 1960, Oxygen transport through hemoglobin solutions. Science 131: 585–590.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • L. Hoofd
    • 1
  • A. Lamboo
    • 1
  1. 1.Department of PhysiologyUniversity of NijmegenNijmegenThe Netherlands

Personalised recommendations