Multicomponent Analysis of Reflection Spectra From The Guinea Pig Heart for Measuring Tissue Oxygenation By Quantitative Determination of Oxygen Saturation of Myoglobin and of The Redox State of Cytochrome aa3, c, and b
The oxygen saturation of myoglobin can be used as a quantitative indicator of the cellular oxygen supply3,11 as well as of the intracellular oxygen pressure12. On the hemoglobin free perfused Langendorff-heart preparation such measurements can be carried out non-invasively by reflection fotometry4,6,9,10,12 However, our investigations have shown, that under such conditions the usually applied two wavelength method may yield erroneous results 1) because of the superposition of the spectra of myoglobin and cytochromes and 2) the complicated optical conditions during reflection within the tissue4,5. Therefore a larger spectral range is monitored and a new multicomponent evaluation method applied which considers scattering using the two-flux theory of Kubelka and Munk (two-flux evaluation method for multicomponent systems: TEMMS5). The aim of the following experiments was to test the new method under relatively simple and reproducible conditions. These were achieved by arresting and cooling the heart. The results demonstrate quite good applicability of the new evaluation method.
KeywordsReflection Spectrum Heart Surface Multicomponent Analysis Plenum Press Large Spectral Range
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