A New Histochemical Stain for Intracellular Oxygen
The observation of Vaughan and Weberl that oxygen concentrations in the physiological range would quench the fluorescence of pyrenebutyric acid in solution suggested to us that this principle might be used to measure intracellular PO2. Such a technique could eliminate the disadvantages encountered with techniques using electrodes, namely tissue damage and alteration of oxygen tensions by consumption of oxygen, providing that the following criteria could be fulfilled: (a) Penetration of the fluorescent probe into the cell. (b) Absence of metabolic damage as determined by respiration rate. (c) The quenching of the fluorescence of the intracellular probe by oxygen should occur according to a known relationship, preferably Stern-Volmer2.
KeywordsRespiration Rate Fluorescent Probe Oxygen Tension Potassium Phosphate Intracellular Concentration
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