A New Histochemical Stain for Intracellular Oxygen

  • I. S. Longmuir
  • J. A. Knopp
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 37 A)


The observation of Vaughan and Weberl that oxygen concentrations in the physiological range would quench the fluorescence of pyrenebutyric acid in solution suggested to us that this principle might be used to measure intracellular PO2. Such a technique could eliminate the disadvantages encountered with techniques using electrodes, namely tissue damage and alteration of oxygen tensions by consumption of oxygen, providing that the following criteria could be fulfilled: (a) Penetration of the fluorescent probe into the cell. (b) Absence of metabolic damage as determined by respiration rate. (c) The quenching of the fluorescence of the intracellular probe by oxygen should occur according to a known relationship, preferably Stern-Volmer2.


Respiration Rate Fluorescent Probe Oxygen Tension Potassium Phosphate Intracellular Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    W. M. Vaughan and G. Weber, Biochemistry, 9 (1970) 464.PubMedCrossRefGoogle Scholar
  2. 2.
    Stern and M. Volmer, Z. Phys., 20 (1919) 183.Google Scholar
  3. 3.
    I. S. Longmuir, Biochim. J., 64 (1957) 378.Google Scholar
  4. 4.
    I. S. Longmuir and J. A. Knopp, Fed. Proc., 31 (1972) 365.Google Scholar
  5. 5.
    J. Knopp and G. Weber, J. Biol. Chem., 242 (1967) 1353.Google Scholar
  6. 6.
    J. Knopp and I. S. Longmuir, Biochim. Biophys. Acta, 279 (1972) 393.CrossRefGoogle Scholar
  7. 7.
    I. S. Longmuir and F. Allen, J. Polarograph. Soc., VIII (1961) 63.Google Scholar

Copyright information

© Springer Science+Business Media New York 1973

Authors and Affiliations

  • I. S. Longmuir
    • 1
  • J. A. Knopp
    • 1
  1. 1.Department of BiochemistryNorth Carolina State UniversityRaleighUSA

Personalised recommendations