Spectrophotometric Examination of Tissue Oxygenation

  • D. W. Lübbers
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 37 A)


Methods of absorption photometry are sensitive enough to measure natural pigments participating in oxygen supply, as hemoglobin, myoglobin and cytochromes in vivo. The influence of the light on the pigment can be mostly made so small that it can be neglected. Therefore, the history of in vivo measurements is rather long. We might recall the direct measurements performed by G.A. MacMunn (1880), D. Keilin and O. Warburg in the twenties. The results they obtained were very important, but they were mostly qualitative ones and the absolute evaluation remained a difficult problem. Later on, in the thirties, K. Kramer, K. Matthes and G.A. Millikan tried to overcome these difficulties by using the two-wavelengths method measuring on isolated vessels or on earlobe or partially isolated muscle, but all these methods required after each change of the preparation a new calibration with an absolute method. In the fifties, the two-wavelengths method was very much improved by B. Chance.


Reflexion Spectrum Extinction Spectrum Unknown Component Natural Pigment Photometric System 
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Copyright information

© Springer Science+Business Media New York 1973

Authors and Affiliations

  • D. W. Lübbers
    • 1
  1. 1.Max-Planck-Institut für SystemphysiologieDortmundWest Germany

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