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The Oxygen Micro-Electrode

  • I. A. Silver
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 37 A)

Abstract

The reduction of oxygen in a watery solution at a noble metal surface by a low voltage was first observed by Danneel in Nernst’s laboratory in 1897. The application of this technique for biological purposes was instigated by Blinks and Skow (1938) in suspensions of plant cells and was developed for use in mammalian tissues by Davies and Brink (1942). These latter authors made a variety of electrodes which included small elec- trodes of 25 gm platinum wire enclosed in glass insulation which protruded a short distance beyond the end of the platinum. They called these ‘recessed’ micro-electrodes and successfully used them for measuring oxygen gradients on the surface of the brain of the cat. This type of electrode corresponded in its behaviour to the law of classical linear diffusion during its initial polarisation and was able to take its oxygen from very small regions. The disadvantage of this system was that the recess of the electrode was relatively long and readings could only be made at intervals of many minutes. However the readings that were obtained had real meaning and indicated that

Keywords

Pyridine Nucleotide Foreign Body Response Biological Purpose Noble Metal Surface Oxygen Microelectrode 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bicher, H.I. and Knisely, M.H. (1970). J. appl. Physiol. 28, 387.PubMedGoogle Scholar
  2. Blinks, L.R. and Skow, R.K. (1938). Proc. Nat. Acad.Sci. U.S. 24, 420.Google Scholar
  3. Cater, D.B., Phillips, A.F. and Silver, I.A. (1957). Proc. Roy. Soc. B146, 289.Google Scholar
  4. Cater, D.B. and Silver, I.A. (1961). In ‘Reference Electrodes’. (Eds. Ives, D.J.G. and Janz, J.G.). ( Academic Press, London.) Chap. 11.Google Scholar
  5. Cater, D.B., Silver, I.A. and Wilson, G.M. (1959). Proc. Roy. Soc. B. 151, 256.Google Scholar
  6. Chance, B., Legallais, V. and Schoener, B. (1962). Nature 195, 1073.PubMedCrossRefGoogle Scholar
  7. 14.
    Instrumentation and MethodsGoogle Scholar
  8. Clark, L.C. (1956). Amer. Soc. Art. Int. Org. 2, 41.Google Scholar
  9. Danneel, H. (1897–8). Z. Elektrochem. 4, 227.Google Scholar
  10. Davies, P.W. and Brink, F. (1942). Rev. Sci. Instrum. 12, 524.Google Scholar
  11. Davies, P.W. and Rémond, A. (1947). Res. Publ. Assoc.Res. Nerv. Ment. Dis. 26, 205.Google Scholar
  12. Erdmann, W., Kunke, St., Krell, W. (1973). In ‘Oxygen Supply’ (Eds. Kessler, M. et al.). ( Urban and Schwarzen- berg, München. ) p. 169.Google Scholar
  13. Fatt, I. (1964). J. appl. Physiol. 1a, 326.Google Scholar
  14. Grunewald, W. (1973). In ‘Oxygen Supply’ (Eds.Kessler, M. et al.). (Urban and Schwarzenberg,München)p. 160.Google Scholar
  15. Kunze, K. (1965). Pflügers Arch. ges. Physiol. 283, 4.Google Scholar
  16. Kunze, K. (1966). Pflügers Arch. ges. Physiol. 292, 151.Google Scholar
  17. Kunze, K. (1969). In ‘Schriftenreihe Neurologie Ser.Vol. 3. ( Springer, Berlin ).Google Scholar
  18. Lubbers, D.W. and Baumgärtl, H. (1967). Pflügers Arch. ges. Physiol 294, R. 39.Google Scholar
  19. Montgomery, H.and Horwitz, 0. (1950). J. Clin. Invest. 29, 1120.Google Scholar
  20. Silver, I.A. (1965). Med. Electron. Biol. Engn. 377.Google Scholar
  21. Silver, I.A. (1973). In ‘Chemical Engineering and Medicine’. (Advances in Chemistry Series. In press).Google Scholar
  22. Whalen, W.J. and Nair, P. (1967). Circulat. Res. 21, 251.PubMedCrossRefGoogle Scholar
  23. Whalen, W.J. and Nair, P. (1970). Amer. J. Physiol. 219, 973.Google Scholar
  24. Whalen, W.J., Nair, P. and Buerk, D. (1973). In ‘OxygenSupply’ (Eds. Kessler, M. et al.). Urban and Schwarzenberg, Munchen, p. 199.Google Scholar
  25. Whalen, W.J., Riley, J. and Nair, P. (1967). J. appl.Physiol. 23, 798.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1973

Authors and Affiliations

  • I. A. Silver
    • 1
  1. 1.Department of PathologyUniversity of BristolEngland

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