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Differential Anodic Enzyme Polarography for the Measurement of Glucose

  • Leland C. ClarkJr.
  • Eleanor W. Clark
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 37 A)

Abstract

The coupling of enzymes to polarographic and other electrochemical sensors has been a happy one (1–7). The first such union occurred in 1962 (8). The electrochemistry involved lends simplicity to the detecting equipment while in the sensor itself the enzyme provides the specificity, often a degree of specificity which cannot be obtained by any other means. In addition, and very importantly this type of sensor lends itself to miniaturization to the point where it may be used intracellularly.

Keywords

Glucose Oxidase GLUCONIC Acid Enzyme Electrode Yellow Spring Instrument British Patent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Clark, L. C., Jr, A polarographic enzyme electrode for the measurement of oxidase substrates. In: Oxygen Supply, edited by Manfred Kessler, et. al. Munchen-Berlin-Wien, Germany: Urban & Schwarzenberg, 120 - 128 (1973).Google Scholar
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    Clark, L. C. Jr. Membrane polarographic electrode system and method with electrochemical compensation. U.S. Patent No. 3,539,455, issued November 10, 1970.Google Scholar
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    Clark, L. C., Jr. Method of an apparatus for polarographic analysis. British Patent No. 1,167,317, issued October 15, 1969. Improvements in or relating to apparatus for electrochemical analysis. British Patent No. 1,169,140, issued October 15, 1969.Google Scholar
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    The enzyme electrode analytical instrument used in this research is a prototype. It is about to go into production as Model 23 and is available from the Yellow Springs Instrument Co., Yellow Springs, Ohio 45387, U.S.A.Google Scholar
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    Chance, B. The properties of the enzyme-substrate compounds of horseradish peroxidase and peroxides. IV. The effect of pH upon the rate of reaction complex II with several acceptors and its relation to their oxidation-reduction potential. Archives of Biochemistry 24, 410 - 421 (1949).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1973

Authors and Affiliations

  • Leland C. ClarkJr.
    • 1
  • Eleanor W. Clark
    • 1
  1. 1.Children’s Hospital Research FoundationUniversity of Cincinnati College of MedicineUSA

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