Differential Anodic Enzyme Polarography for the Measurement of Glucose

  • Leland C. ClarkJr.
  • Eleanor W. Clark
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 37 A)


The coupling of enzymes to polarographic and other electrochemical sensors has been a happy one (1–7). The first such union occurred in 1962 (8). The electrochemistry involved lends simplicity to the detecting equipment while in the sensor itself the enzyme provides the specificity, often a degree of specificity which cannot be obtained by any other means. In addition, and very importantly this type of sensor lends itself to miniaturization to the point where it may be used intracellularly.


Glucose Oxidase GLUCONIC Acid Enzyme Electrode Yellow Spring Instrument British Patent 
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  1. 1.
    Clark, L. C., Jr, A polarographic enzyme electrode for the measurement of oxidase substrates. In: Oxygen Supply, edited by Manfred Kessler, et. al. Munchen-Berlin-Wien, Germany: Urban & Schwarzenberg, 120 - 128 (1973).Google Scholar
  2. 2.
    Clark, Leland C., Jr. A family of polarographic enzyme electrodes and the measurement of alcohol. Biotechnology & Bioengineering Symposium No. 3, 377 - 394 (1972).Google Scholar
  3. 3.
    Clark, L. C. Jr. Membrane polarographic electrode system and method with electrochemical compensation. U.S. Patent No. 3,539,455, issued November 10, 1970.Google Scholar
  4. 4.
    Clark, L. C., Jr. Method of an apparatus for polarographic analysis. British Patent No. 1,167,317, issued October 15, 1969. Improvements in or relating to apparatus for electrochemical analysis. British Patent No. 1,169,140, issued October 15, 1969.Google Scholar
  5. 5.
    Clark, L. C., Jr. Method and apparatus for polarographic analysis. Japanese Letters Patent No. 610,201, issued November 11, 1970.Google Scholar
  6. 6.
    Clark, L. C., Jr. Future horizons of analytical electrochemistry in the neurophysiology of mental retardation. American Journal of Mental Deficiency 77 (5), 633 - 634 (1973).PubMedGoogle Scholar
  7. 7.
    Guilbault, C. G. (Ed.) Enzymatic Methods of Analysis. Oxford, England: Pergamon Press (1970).Google Scholar
  8. 8.
    Clark, L. C., Jr. and Champ Lyons. Electrode systems for continuous monitoring in cardiovascular surgery. Annals of the New York Academy of Sciences 102, 29 - 45 (1962).PubMedCrossRefGoogle Scholar
  9. 9.
    The enzyme electrode analytical instrument used in this research is a prototype. It is about to go into production as Model 23 and is available from the Yellow Springs Instrument Co., Yellow Springs, Ohio 45387, U.S.A.Google Scholar
  10. 10.
    Cooper, G. R. and V. McDaniel. Methods for the determination of glucose. Public Health Service, Atlanta, Georgia (1966).Google Scholar
  11. 11.
    George Sachs. Bioelectrodes for tissue metabolism Clark, L. C., Jr. and George Sachs. Bioelectrodes for tissue metabolism. Conference on Bioelectrodes. Annals of the New York Academy of Sciences 148, 133 - 153 (1968).PubMedCrossRefGoogle Scholar
  12. 12.
    Hicks, G. P. and S. J. Updike. Enzyme electrode. U.S. Patent No. 3,542,622, issued November 24, 1970.Google Scholar
  13. 13.
    Hedin, G. G., J. E. Johansson and T. Thunberg, editors. Hammarsten,O: Lehrbuch der Physiologischen Chemie, Chapter 5 entitled Das Blut, “Glucose in erythrocytes and plasma; p. 264. Munchen und Wiesbaden: J. F. Bergmann (1922).Google Scholar
  14. 14.
    Clark, L. C., Jr., George Misrahy and R. Phyllis Fox. Chronically implanted polarographic electrodes. Journal of Applied Physiology 13, 85 - 91 (1958).PubMedGoogle Scholar
  15. 15.
    Chance, B. The properties of the enzyme-substrate compounds of horseradish peroxidase and peroxides. IV. The effect of pH upon the rate of reaction complex II with several acceptors and its relation to their oxidation-reduction potential. Archives of Biochemistry 24, 410 - 421 (1949).PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1973

Authors and Affiliations

  • Leland C. ClarkJr.
    • 1
  • Eleanor W. Clark
    • 1
  1. 1.Children’s Hospital Research FoundationUniversity of Cincinnati College of MedicineUSA

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