Introduction of Artificial Crosslinks into Proteins

  • Rosa Uy
  • Finn Wold
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 86A)


In this chapter, we present a brief overview of the current status of protein crosslinking technology. An attempt is made to compare the natural crosslinks and natural crosslinking agents to the artificial ones, and a brief section is devoted to the potential use of enzymes (transglutaminase and peroxidase) as cross-linking agents in vitro. Homobifunctional (x-R-x) and heterobifunctional (x-R-y) reagents are considered in terms of the kinds of functional groups and R-groups that have been used in protein crosslinking, and some examples of reagents and applications from the recent literature are tabulated.


Cross Link Crosslink Formation Zero Length Intermolecular Crosslinks Bifunctional Reagent 


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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Rosa Uy
    • 1
  • Finn Wold
    • 1
  1. 1.Department of BiochemistryUniversity of MinnesotaSt. PaulUSA

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