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Identification of Binding Sites on the E. Coli Ribosome by Affinity Labeling

  • Barry S. Cooperman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 86A)

Abstract

Both electrophilic and photolabile derivatives of several different types of ribosomal ligands have been used in affinity labeling studies on the Escherichia coli ribosome. These studies have resulted in the localization of the peptidyl transferase center within a region of the 50S subunit, and the localization of the mRNA binding site within one of two regions on the 30S particle. in addition, labeling data have been obtained for GTP and streptomycin affinity labels. The affinity labeling results are discussed along with the results of other studies, and procedures are suggested for improving the resolving power of the affinity labeling technique as applied to ribosomes.

Keywords

Ribosomal Protein Ribosome Binding Site Photoaffinity Label Peptidyl Transferase Peptidyl Transferase Center 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Barry S. Cooperman
    • 1
  1. 1.Department of ChemistryUniversity of PennsylvaniaPhiladelphiaUSA

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