Thiolation and Disulphide Cross-Linking of Insulin to Form Macromolecules of Potential Therapeutic Value
Macromolecules have been prepared containing native insulin carried by a modified insulin skeleton made by partially thiolating the insulin hexamer and forming intermolecular cross-links through disulphide bridges. Oxidation of partially thiolated insulin (0.5 – 0.7 SH group/mole), formed by reacting insulin with AHTL, with, (a) potassium ferricyanide, (b) Cu++-oxygen gave water soluble macromolecules containing 20–26 and 410–708 monomer units respectively which had rod-random coil shape (light scattering). the larger molecules formed by (b) contained 8g-atom Cu++/hexamer unit and insulin. The insulin was firmly bound within the macromolecules and was probably bound within an insulin-modified insulin hexamer through coordination to copper.
KeywordsDisulphide Bond Potassium Ferricyanide Disc Electrophoresis Cupric Chloride Native Insulin
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