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Synthesis and Application of New Bifunctional Reagents

  • Wolfgang E. Trommer
  • Klaus Friebel
  • Hans-Hermann Kiltz
  • Hans-Jörg Kolkenbrock
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 86A)

Summary

Two new bifunctional reagents suited for the step-wise cross-linking of cysteine and lysine residues in proteins are described. Application to lactate dehydrogenase yields a cross-link between cysteine-165 and lysine-179, which suggests an alternative mechanism by which the “essential” cysteine reacts. for the mapping of the environment of a known and well defined amino acid the use of semireversible bifunctional reagents is suggested.

Keywords

Lactate Dehydrogenase Anchor Point Maleic Anhydride Amino Acid Side Chain Excess Reagent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Wolfgang E. Trommer
    • 1
  • Klaus Friebel
    • 1
  • Hans-Hermann Kiltz
    • 1
  • Hans-Jörg Kolkenbrock
    • 1
  1. 1.Lehrstuhl für Biochemie, Abteilung ChemieRuhr-Universität BochumBochumWest Germany

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