Effects of Neomycin on Polyphosphoinositides in Inner Ear Tissues and Monomolecular Films

  • J. Schacht
  • S. Lodhi
  • N. D. Weiner
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 84)


The effect of neomycin on polyphosphoinositides was studied in vivo and in vitro. In vivo, the incorporation of 32 Pi into phosphatidylinositoi phosphate and phosphatidylinositol diphosphate was measured in inner ear tissues. Concentrations of neomycin which decreased the electrophysiological response of the cochlea to sound stimulation also decreased labeling of phosphatidylinositol diphosphate. In vitro experiments with brain tissues and polyphosphoinositide extracts indicated a direct interaction between the lipids and neomycin.

Neomycin interacts strongly with monomolecular films of polyphosphoinositides. The interaction appears to be complex and is a function of neomycin concentration in the subphase and surface pressure of the film. Condensation of the polyphosphoinositide film is favored at low neomycin concentrations and low film pressures while expansion of the film is favored at high neomycin concentrations and high film pressures. The interactions of neomycin with other negatively charged films (phosphatidyl inositol and phosphatidyl serine) are much weaker, particularly at low neomycin concentrations.

The metabolic and physiological consequences of the neomycin/polyphosphoinositide interaction are discussed in regard to the ototoxicity of the drug.


Phosphatidyl Inositol Ethacrynic Acid Acidic Phospholipid Lipid Label Phosphatidylinositol Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • J. Schacht
    • 1
  • S. Lodhi
    • 2
  • N. D. Weiner
    • 2
  1. 1.Kresge Hearing Research InstituteUniversity of MichiganAnn ArborUSA
  2. 2.College of PharmacyUniversity of MichiganAnn ArborUSA

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