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Diffusional Transport of Toxic Materials in Membranes Studied by Fluorescence Spectroscopy

  • Joseph R. Lakowicz
  • Delman Hogan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 84)

Abstract

As a nation we are currently concerned with the effects of a multitude of synthetic chemicals on life processes. The diversity of opinions on these issues reflects, in part, a lack of understanding of the molecular aspects of toxicity and bioaccumulation. Integral to these concerns is the effect of toxic materials on cell membranes and the permeability barriers which these membranes impose to xenobiotics.

Fluorescence spectroscopy provides a powerful tool for investigating many aspects of membrane sensitivity to toxic materials. Chlorinated hydrocarbons, olefins, and amines act as diffusional quenchers of fluorescence. Measurement of the fluorescence lifetimes of probes embedded in biological membranes can reveal the probe-quencher collisional frequency, and hence the xenobiotic’s diffusion coefficient in the membrane. Such information, coupled with the rates of exchange of foreign materials between serum proteins and membranes, may possibly allow predictions of the bioaccumulation potential of toxic materials.

Fluorescence quenching studies can also be used to determine the xenobiotic’s membrane-water partition coefficient. A range of values from 10 to 108 appear to be experimentally accessible. Localization of foreign materials in either the glycerol or acyl side chain region of a membrane may be revealed by investigations using localized fluorescent probes. In favorable circumstances it appears likely that one can measure both the xenobiotic’s lateral diffusion rate across the membrane’s surface and the transport rate through the bilayer.

Keywords

Fluorescence Quenching Fluorescence Lifetime DIFFUSIONAL Transport Quencher Concentration Toxic Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature

  1. 1.
    BARENBOIM, G.M. 1963. Interaction of Excited Biomolecules with Oxygen-1. Quenching of the Photoluminescence of Biomole-cul es by Oxygen and Nitric Oxide. Biofizika 8: 154–164.Google Scholar
  2. 2.
    BIERI, V.G., and HOELZYL WALLACH, D.F. 1975. A Study Using Paramagnetic Quenching of Fluorescence by Nitroxide Lipid Analogues. Biochim. Biophys. Acta 406: 415–423.PubMedCrossRefGoogle Scholar
  3. 3.
    BOWEN, E.J., and METCALF, W.S. 1951. The Quenching of Anthracene Fluorescence. Proc. Roy. Soc.(London) A 206: 437–447.CrossRefGoogle Scholar
  4. 4.
    CHEN, R.F. 1971. Fluorescence Quenching Due to Mercuric Ion. Interaction with Aromatic Amino Acids and Proteins. Arch. Biochem. Biophys. 142: 552–564.PubMedCrossRefGoogle Scholar
  5. 5.
    COGAN, V., SHINITZKY, M., WEBER, G., and NISHIDA, T. 1973. Microviscosity and Order in the Hydrocarbon Region of Phospholipid and Phospholipid-cholesterol Dispersions Determined with Fluorescent Probes. Biochemistry 12: 521–529.PubMedCrossRefGoogle Scholar
  6. 6.
    HORROCKS, A.R., KEARVELL, A., TICKLE, K., and WILKINSON, F. 1966. Mechanism of Fluorescence Quenching in Solution. Part 2-Quenching by Xenon and Intersystem Crossing Efficiencies. Trans. Faraday Soc. 62: 3393–3399.CrossRefGoogle Scholar
  7. 7.
    KARIM AHMED, A. 1976. Environment 18: 6.CrossRefGoogle Scholar
  8. 8.
    KATES, M. 1972. Techniques in Lipidology. Isolation, Analysis and Identification of Lipids. North-Holland Publishing Co., Amsterdam.Google Scholar
  9. 9.
    KAUTSKY, H. 1939. Quenching of Luminescence by Oxygen. Trans. Faraday Soc. 35: 216–226.CrossRefGoogle Scholar
  10. 10.
    KIRKWOOD, S., and PHILLIPS, P.H. 1946. The Relationship Between the Lipoid Affinity and the Insecticidal Action of 1,1-bis (p-fluorophenyl) 2,2.2-trichloroethane and Related Substances. J. Pharmacology 87: 375–381.Google Scholar
  11. 11.
    KORNBERG, R.D., and McCONNELL, H.M. 1971. Inside-Outside Transitions of Phospholipids in Vesicle Membranes. Biochemistry 10: 1111–1120.PubMedCrossRefGoogle Scholar
  12. 12.
    LAKOWICZ, J.R. Unpublished Observations.Google Scholar
  13. 13.
    LEE, A.G., BIRDSALL, J.M., METCALFE, J.C. 1973. Measurement of Fast Lateral Diffusion of Lipids in Vesicles and Biological Membranes by 1H Nuclear Magnetic Resonance. Biochemistry 12: 1650–1658.PubMedCrossRefGoogle Scholar
  14. 14.
    LENARD, J and ROTHMAN, J.L. 1976. Transbilayer Distribution and Movement of Cholesterol and Phospholipid in the Membrane of Influenze Virus. Proc. Natl. Acad. Sci. 73: 391–395.PubMedCrossRefGoogle Scholar
  15. 15.
    LEONHARDT, H., and WELLER, A. 1961. Fluorescence Quenching Studied by Flash Spectroscopy. In: Luminescence of Organic and Inorganic Materials. Hartmeet, Kallman and Grace (Eds.) Wiley, N.Y.Google Scholar
  16. 16.
    MATAGA, N., OKADA, T., and EZUMI, K. 1966. Fluroescence of Pyrene-N,N-dimethylaniline Complex in Non-polar Solvent. Mol. Phys. 10: 203–204.CrossRefGoogle Scholar
  17. 17.
    MEDINGER, T., and WILKINSON, F. 1965. Mechanism of Fluorescence Quenching in Solution Part I. Quenching by Bromobenzene. Trans. Faraday. Soc. 61: 620–630.CrossRefGoogle Scholar
  18. 18.
    MELNIKOV, N.N. 1971 Chemistry of Pesticides. Springer Verlag, N.Y. Chapter 5: 75.CrossRefGoogle Scholar
  19. 19.
    ROLLEFSON, G.K., and BOAZ, H. 1948. Quenching of Fluorescence in Solution. J. Phys. Colloid Chem. 52: 518–527.PubMedCrossRefGoogle Scholar
  20. 20.
    SCANDELLA, C.J., DEVAUX, P. and McCONNELL, H.M. 1972. Rapid Lateral Diffusion of Phospholipids in Rabbit Sarcoplasmic Reticulum. Proc. Nat. Acad. Sci. U.S.A. 69: 2056–2060.CrossRefGoogle Scholar
  21. 21.
    STICKEL, L.F. 1973. Pesticide Residues in Birds and Mammals, Chapter 7. Environmental Pollution by Pesticides (Edwards, C.A., Ed.). Plenum Press, NY.Google Scholar
  22. 22.
    SVESHNIKOFF, B. 1936. The Quenching of Fluorescence of Dye Solutions by Foreign Compounds. Acta Physiochimica U.R.S.S. IV: 453–470.Google Scholar
  23. 23.
    TSONG, T.Y. 1975. Effect of Phase Transition on the Kinetics of Dye Transport in Phospholipid Bilayer Structures. Biochemistry 14: 5409–5414.PubMedCrossRefGoogle Scholar
  24. 24.
    TURI, J.S., HO, N.F.H., HIGUCHI, W.I., and SHIPMAN, C. Jr. 1975. Systems Approach to Study of Solute Transport Across Membranes Using Suspension Cultures of Mammalian Cells. III: Steady-state Diffusion Models. J. Pharm. Sci. 64: 622–626.PubMedCrossRefGoogle Scholar
  25. 25.
    WAGGONER, A.S. and STRYER, L. 1970. Fluorescent Probes of Biological Membranes. Proc. Natl. Acad. Sci. 67: 579–589.PubMedCrossRefGoogle Scholar
  26. 26.
    WARE, W.R. and NOVROS, J.S. 1966. Kinetics of Diffusion-Con­trolled Reactions. An Experimental Test of the Theory as Applied to Fluorescence Quenching. J. Phys. Chem. 70: 3246–3253.CrossRefGoogle Scholar
  27. 27.
    WARE, W.R., WATT, D., and HOLMES, J.D. 1974. Exciplex Photo- physics. I. The a-Cyano-Naphthalene-Olefin System. J. Amer. Chem. Soc. 96: 7853–7860.CrossRefGoogle Scholar
  28. 28.
    WEISS, J. 1939. Photosensitized Reactions and the Quenching of Fluorescence in Solutions. Trans. Faraday Soc. 35: 48–64.CrossRefGoogle Scholar
  29. 29.
    ZIMMERMAN, O.T. 1946. DDT, Killer of Killers. Industrial Research Service, Dover, NH.CrossRefGoogle Scholar
  30. 30.
    ZINSSER, H. 1935. Rats, Lice, and History. Boston Little Brown, 88Google Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Joseph R. Lakowicz
    • 1
  • Delman Hogan
    • 1
  1. 1.Freshwater Biological InstituteUniversity of MinnesotaNavarreUSA

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