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Lead Actions on Sodium-Plus-Potassium-Activated Adenosinetriphosphatase from Electroplax, Rat Brain, and Rat Kidney

  • George J. Siegel
  • Suzanne K. Fogt
  • Mary Jane Hurley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 84)

Abstract

Inorganic lead ion, in micromolar concentrations, reversibly inhibits the sodium-plus-potassium-activated adenosinetriphosphatase (ATPase) and potassium-activated p-nitrophenylphosphatase (NPPase) activities of microsomal fractions from electric organ, rat kidney, and rat brain. In the presence of 3 mM MgCl2 and 3 mM ATP, the concentrations of PbCl2 producing half-maximal inhibition of the ATPase from these tissues are 4 × 10-6 M, 20 × 10-6 M, and 55 × 10-6 M, respectively. The corresponding values for inhibition of the NPPase are 10-6 M, 53 × 10-6 M, and 22 × 10-6 M. PbCl2 also stimulates the phosphorylation by [γ-32P]ATP of a microsomal protein from all three tissues in the absence of added sodium ion. This reaction -was extensively studied with electroplax microsomes. In common -with the well-known Na+-dependent phosphorylation of CNa+ + K+)-ATPase, the Fb2 -dependent reaction is inhibited by ouabain, specific for ATP, dependent on Mg2+, and yields an acid-stable phosphoprotein with a molecular weight of 98,000 in sodium dodecylsulfate. The Pb2+-dependent phosphoprotein, however, is not sensitive to K+. These observations are pertinent to the biochemistry and toxicity of inorganic lead in tissues and to the molecular mechanism of the cation transport enzyme.

Keywords

ATPase Activity Lead Poisoning Electric Organ Adenosine Triphosphatase Dependent Phosphorylation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • George J. Siegel
    • 1
  • Suzanne K. Fogt
    • 1
  • Mary Jane Hurley
    • 1
  1. 1.Neurology Research Laboratory, Neurology DepartmentUniversity of Michigan Medical CenterAnn ArborUSA

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