Effect of P-Chloromercuribenzoate (pCMB), Ouabain and 4-Acetamido-4′ISO-Thiocyamatostilbene-2,2′-Disulfonic Acid (Sits) on Proximal Tubular Transport Processes
Using microperfusion techniques the following transport parameters of the proximal tubule were measured: 1. Isotonic fluid (Na+) absorption (JNa). 2. Zero net flux concentration (electrochemical potential) differences which are proportional to the respective active transport rates of H+ Cglycodiazine), D-glucose (α-methyl-D-glycoside), L-histidine, inorganic phosphate and calcium ions. 3. Transtubular and transcellular electrical potential differences and transcellular resistances.
Ouabain (1mM) applied peritubularly in golden hamsters inhibited JNa incompletely and the sodium-coupled (secondary active) transport processes of glucose, histidine, phosphate and Ca++ by more than 80%. The H+ (glycodiazine) transport was not affected. Ouabain (1mM) plus acetazolamide (0.2 mM) inhibited JNa completely.
In the rat, pCMB (0.2 mM) when applied long enough, inhibits JNa completely. At a time of pCMB application when JNa is reduced to 1/3, this substance inhibits the active H+ (glycodiazine) transport which must be considered to be a direct action of pCMB. Furthermore it inhibits the secondary active phosphate transport either directly or via the inhibition of Na+ and/or H+ transport. The secondary active glucose, histidine and Ca++ transport are little affected by pCMB. pCMB reduces the cell potential, reversibly, yet leaves unchanged the resistance ratio of the luminal to peritubular cell membrane .
In the rat SITS inhibits JNa moderately but the active H+ (glycodiazine) transport strongly. It does not affect the glucose transport.
On the basis of these and other results a hypothesis of the interaction of Na+ and H+ (HCO3 -) transport is given.
KeywordsProximal Tubule Golden Hamster Resistance Ratio Electrical Potential Difference Capillary Perfusate
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