Effect of P-Chloromercuribenzoate (pCMB), Ouabain and 4-Acetamido-4′ISO-Thiocyamatostilbene-2,2′-Disulfonic Acid (Sits) on Proximal Tubular Transport Processes

  • K. J. Ullrich
  • G. Capasso
  • G. Rumrich
  • K. Sato
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 84)


Using microperfusion techniques the following transport parameters of the proximal tubule were measured: 1. Isotonic fluid (Na+) absorption (JNa). 2. Zero net flux concentration (electrochemical potential) differences which are proportional to the respective active transport rates of H+ Cglycodiazine), D-glucose (α-methyl-D-glycoside), L-histidine, inorganic phosphate and calcium ions. 3. Transtubular and transcellular electrical potential differences and transcellular resistances.

The following was found:
  1. 1.

    Ouabain (1mM) applied peritubularly in golden hamsters inhibited JNa incompletely and the sodium-coupled (secondary active) transport processes of glucose, histidine, phosphate and Ca++ by more than 80%. The H+ (glycodiazine) transport was not affected. Ouabain (1mM) plus acetazolamide (0.2 mM) inhibited JNa completely.

  2. 2.

    In the rat, pCMB (0.2 mM) when applied long enough, inhibits JNa completely. At a time of pCMB application when JNa is reduced to 1/3, this substance inhibits the active H+ (glycodiazine) transport which must be considered to be a direct action of pCMB. Furthermore it inhibits the secondary active phosphate transport either directly or via the inhibition of Na+ and/or H+ transport. The secondary active glucose, histidine and Ca++ transport are little affected by pCMB. pCMB reduces the cell potential, reversibly, yet leaves unchanged the resistance ratio of the luminal to peritubular cell membrane .

  3. 3.

    In the rat SITS inhibits JNa moderately but the active H+ (glycodiazine) transport strongly. It does not affect the glucose transport.


On the basis of these and other results a hypothesis of the interaction of Na+ and H+ (HCO3 -) transport is given.


Proximal Tubule Golden Hamster Resistance Ratio Electrical Potential Difference Capillary Perfusate 
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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • K. J. Ullrich
    • 1
  • G. Capasso
    • 1
  • G. Rumrich
    • 1
  • K. Sato
    • 1
  1. 1.Max-Planck-Institut für BiophysikFrankfurt/MainGermany

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