Effect of P-Chloromercuribenzoate (pCMB), Ouabain and 4-Acetamido-4′ISO-Thiocyamatostilbene-2,2′-Disulfonic Acid (Sits) on Proximal Tubular Transport Processes

  • K. J. Ullrich
  • G. Capasso
  • G. Rumrich
  • K. Sato
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 84)


Using microperfusion techniques the following transport parameters of the proximal tubule were measured: 1. Isotonic fluid (Na+) absorption (JNa). 2. Zero net flux concentration (electrochemical potential) differences which are proportional to the respective active transport rates of H+ Cglycodiazine), D-glucose (α-methyl-D-glycoside), L-histidine, inorganic phosphate and calcium ions. 3. Transtubular and transcellular electrical potential differences and transcellular resistances.

The following was found:
  1. 1.

    Ouabain (1mM) applied peritubularly in golden hamsters inhibited JNa incompletely and the sodium-coupled (secondary active) transport processes of glucose, histidine, phosphate and Ca++ by more than 80%. The H+ (glycodiazine) transport was not affected. Ouabain (1mM) plus acetazolamide (0.2 mM) inhibited JNa completely.

  2. 2.

    In the rat, pCMB (0.2 mM) when applied long enough, inhibits JNa completely. At a time of pCMB application when JNa is reduced to 1/3, this substance inhibits the active H+ (glycodiazine) transport which must be considered to be a direct action of pCMB. Furthermore it inhibits the secondary active phosphate transport either directly or via the inhibition of Na+ and/or H+ transport. The secondary active glucose, histidine and Ca++ transport are little affected by pCMB. pCMB reduces the cell potential, reversibly, yet leaves unchanged the resistance ratio of the luminal to peritubular cell membrane .

  3. 3.

    In the rat SITS inhibits JNa moderately but the active H+ (glycodiazine) transport strongly. It does not affect the glucose transport.


On the basis of these and other results a hypothesis of the interaction of Na+ and H+ (HCO3 -) transport is given.


Proximal Tubule Golden Hamster Resistance Ratio Electrical Potential Difference Capillary Perfusate 


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  1. 1.
    BAUMANN, K., DEROUFFIGNAC, C., ROINEL, N., RUMRICH, G., ULLRICH, K.J.: Pflügers Arch. 356 (1975) 287.PubMedCrossRefGoogle Scholar
  2. 2.
    EVERS, J., MURER, H., KINNE, R.: Biochim. Biophys. Acta 426 (1976) 598.PubMedCrossRefGoogle Scholar
  3. 3.
    FRÖMTER, E., MüLLER, C.W. and WICK, T.: In Electrophysiology of Epithelial Cells, p. 119 (Giebisch, G., Ed.). F.K. Schattauer. Stuttgart-New York (1971).Google Scholar
  4. 4.
    FRÖMTER, E., RUMRICH, G., ULLRICH, K.J.: Pflügers Arch, 343 (1973) 189.PubMedCrossRefGoogle Scholar
  5. 5.
    FRÖMTER, E., GEßNER, K.: Pflügers Arch. 35 (1974) 85.CrossRefGoogle Scholar
  6. 6.
    FRÖMTER, E. and GEBIER, K.: Pflügers Arch. 357 (1975) 209.PubMedCrossRefGoogle Scholar
  7. 7.
    GMAJ, P. and MURER, H. Personal communication.Google Scholar
  8. 8.
    HOFFMANN, N., THEES, M., KINNE, R.: Pflügers Arch. 262 (1976) 147.CrossRefGoogle Scholar
  9. 9.
    KINNE-SAFFRAN, E., KINNE, R.: Proc. Soc. exp. Biol.Med. 146 (1974) 751.PubMedGoogle Scholar
  10. 10.
    KINNE, R., MURER, H., KINNE-SAFFRAN, E., THEES, M., SACHS, G.: J. Membrane Biol. 21 (1975) 375.CrossRefGoogle Scholar
  11. 11.
    KLEINZELLER, A., CORT, J.H.: Biochem. J. 67 (1957) 15.PubMedGoogle Scholar
  12. 12.
    MURER, H., HOPFER, U., KINNE, R.: Biochem. J. 154 (1976) 597.PubMedGoogle Scholar
  13. 13.
    SAMARZIJA, I., FRÖMTER, E.: Pflügers Arch. 359 (1975) R 119.Google Scholar
  14. 14.
    ULLRICH, K.J., FRÖMTER, E., BAUMANN, K.: In Laboratory Technique in Membrane Biophysics, p. 106 (Passow, H., Stampfli, R., Eds.). Springer. Heidelberg (1969).CrossRefGoogle Scholar
  15. 15.
    ULLRICH, K.J., RADTKE, H.W., RUMRICH, G.: PflÖgers Arch. 330 (1971) 149.PubMedCrossRefGoogle Scholar
  16. 16.
    ULLRICH, K.J., FASOLD, H., KLÖSS, S., Rumrich, G., Salzer, M., Sato, K., Simon, B., deVries, J. X.: Pflügers Arch. 344 (1973) 51.PubMedCrossRefGoogle Scholar
  17. 17.
    ULLRICH, K.J., SATO, K., RUMRICH, G.: Transport Mechanisms in Epithelia, p. 560, (USSSING, H.H., THORN, N.A., Eds.). Munks-gard. Copenhagen (1973).Google Scholar
  18. 18.
    ULLRICH, K.J., RUMRICH, G., KLÖSS, S.: Pflügers Arch. 351 (1974) 35.PubMedCrossRefGoogle Scholar
  19. 19.
    ULLRICH, K.J., RUMRICH, G., KLÖSS, S.: Pflügers Arch. 351 (1974) 49.PubMedCrossRefGoogle Scholar
  20. 20.
    ULLRICH, K.J., RUMRICH, G., BAUMANN, K.: Pflügers Arch. 357 (1975) 149.PubMedCrossRefGoogle Scholar
  21. 21.
    ULLRICH, K.J., RUMRICH, G., KLÖSS, S.: Pflügers Arch. in press.Google Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • K. J. Ullrich
    • 1
  • G. Capasso
    • 1
  • G. Rumrich
    • 1
  • K. Sato
    • 1
  1. 1.Max-Planck-Institut für BiophysikFrankfurt/MainGermany

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