Advertisement

The Specificity of Mammalian Desaturases

  • A. T. James
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 83)

Abstract

Studies of the metabolism of unsaturated fatty acids by mammals, particularly the rat, have been done over many years both by feeding unlabelled fatty acids followed by analytical and structural studies of the resultant tissue fatty acids and by using labelled substrates in whole animals, individual organs and tissue homogenates. Many pathways for individual fatty acids have been elucidated particularly in the essential fatty acid series, but until recently there has been much confusion in the literature as to the number and type of desaturase enzymes. Only in the last few years has there been any attempt at systematic investigation of enzyme-substrate specificity since such work is hampered by the failure to isolate single enzymes. All the desaturases so far studied appear to be particulate of very high molecular weight and cannot be purified by conventional means.

Keywords

Double Bond Unsaturated Acid Dienoic Acid Double Bond Position Monoenoic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Beerthuis, R. K., Nugteren, D. H., Pabon, H. J. J. & van Dorp, D. A. (1968) Recuit 87, 461.CrossRefGoogle Scholar
  2. Brenner, R. R. & Peluffo, R. O. (1966), J. Biol. Chem. 241, 5213.PubMedGoogle Scholar
  3. Brenner, R. R. (1971) Lipids 6, 567.PubMedCrossRefGoogle Scholar
  4. Brett, D., Howling, D., Morris, L. J. & James, A. T. (1971), Arch. Biochem. Biophys. 143, 535.PubMedCrossRefGoogle Scholar
  5. Budny, J. & Sprecher, H. (1971), Biochim. Biophys. Acta 239, 190.PubMedGoogle Scholar
  6. Chang Huei-Che & Holman, R. T. (1970) Biochem. Biophys. Acta 280, 17.Google Scholar
  7. de Iong, H. & Thomasson, H. J. (1956) Nature 175, 1051.CrossRefGoogle Scholar
  8. Deuell, H. J. Jr. & Greenberg, S. M. (1950) Fortschr. Chem. Org. Naturstoffe 6, 1 Wien Springen Verlag.Google Scholar
  9. Fulco, A. J. & Mead, J. F. (1959) J. Biol. Chem. 234, 1411.PubMedGoogle Scholar
  10. Gurr, M. I., Robinson, M. P., James, A. T., Morris, L. J. & Howling, D. (1972) Biochim. Biophys. Acta 280, 415.PubMedGoogle Scholar
  11. Holman, R. T. (1970) Progress Chem. Fats 9, 611.Google Scholar
  12. Howling, D., Morris, L. J., Gurr, M. I. & James, A. T. (1972) Biochim. Biophys. Acta 260, 10.PubMedGoogle Scholar
  13. Hall, S. & James, A. T. (1976) (Unpublished observations)Google Scholar
  14. James, A. T. (1973): in “Current Trends in the Biochemistry of Lipids”, edited by J. Ganguly & R.M.S. Smellie and published by Academic Press, p. 49.Google Scholar
  15. Jacob, J. & Grimmer, G. (1971) Hoppe Seyler’s Z. Physiol. Chem. 352, 1445.PubMedCrossRefGoogle Scholar
  16. Klenk, E. & Tschope, G. (1963) Z. Physiol. Chem. 334, 193.CrossRefGoogle Scholar
  17. Klenk, E. (1965) Adv. in Lipid Res. 3, 1.Google Scholar
  18. Klenk, E. (1963) Z. Physiol. Chem. 331, 50.CrossRefGoogle Scholar
  19. Mead, J. F. & Slaton, W. H. Jr. (1956) J. Biol. Chem. 219, 705.PubMedGoogle Scholar
  20. Mead, J. F. & Howton, D. R. (1957) J. Biol. Chem. 229, 575.PubMedGoogle Scholar
  21. Nugteren, D. H. (1965) Biochim. Biophys. Acta 106, 280.PubMedGoogle Scholar
  22. Pollard, M., Gunstone, F., Morris, L. J. & James, A. T. (1976) (In preparation).Google Scholar
  23. Rahm, J. J. & Holman, R. T. (1964) J. Lipid Res. 5, 169.PubMedGoogle Scholar
  24. Schlenk, H., Gellerman, J. L. & Sand, D. M. (1967) Biochem. Biophys. Acta 137, 420.PubMedGoogle Scholar
  25. Schlenk, H., Sand, D. M. & Sen, N. (1964) Biochim. Biophys. Acta 87, 361.Google Scholar
  26. Schlenk, H., Gerson, T. & Sand, D. M. (1969) Biochim. Biophys. Acta 176, 740.PubMedGoogle Scholar
  27. Schlenk, H., Sand, D. M. & Gellerman, J. L. (1969) Biochim. Biophys. Acta 187, 201.PubMedGoogle Scholar
  28. Schlenk, H. & Sand, D. M. (1967) Biochim. Biophys. Acta 144, 305.PubMedGoogle Scholar
  29. Sprecher, H. (1967a) Lipids 2, 122.CrossRefGoogle Scholar
  30. Sprecher, H. (1967b) Biochim. Biophys. Acta 144, 296.PubMedGoogle Scholar
  31. Sprecher, H. (1968) Lipids 3, 14.PubMedCrossRefGoogle Scholar
  32. Stearns, E. M., Rysary, J. A. & Privett, O.S. (1967) J. Nutr. 93, 485.PubMedGoogle Scholar
  33. Stoffel, W. (1966) Naturwiss 24, 621.CrossRefGoogle Scholar
  34. Stoffel, W. & Scheid, A. (1967) Hoppe Seyler’s Z. Physiol. Chem. 348, 205.PubMedCrossRefGoogle Scholar
  35. Stoffel, W. (1973) Z. Physiol. Chem. 333, 71.CrossRefGoogle Scholar
  36. Thomasson, H. J. (1953) Intern. Z. Vitaminforsch 25, 62.Google Scholar
  37. Thomasson, H. J. (1962) Nature 194, 973.PubMedCrossRefGoogle Scholar
  38. Ullman, D. & Sprecher, H. (1971) Biochim. Biophys. Acta 248, 186.PubMedGoogle Scholar
  39. Verdino, B., Blank, M. L., Privett, O. S. & Lundberg, W. O. (1964) J. Nutr. 83, 234.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • A. T. James
    • 1
  1. 1.Biosciences DivisionUnilever Research Laboratory Colworth/Welwyn Colworth House SharnbrookBedfordEngland, UK

Personalised recommendations