The Role of Lipid in Stearyl CoA Desaturation
The fatty acid desaturase system was one of the first microsomal enzymes for which a lipid requirement was established. Using Fleischer’s procedure of aqueous acetone extraction (Lester & Fleischer, 1961) we were able to reduce the desaturase activity of hen liver microsomes to very low levels and restore activity completely with a mixture of lipids isolated from the microsomes (Holloway & Peluffo, 1964; Jones at al., 1969). Shortly after this observation cytochrome b5 was implicated in the desaturation process (Oshino et al., 1966). Progress in the isolation and purification of the stearyl CoA desaturase has been slow and it is only in the last two years that the complete resolution and reconstitution of the stearyl CoA desaturase has been achieved by Strittmatter and co-workers (Strittmatter et al., 1974).
KeywordsLipid Vesicle Desaturase Activity Vesicle Population Stokes Radius Desaturation Process
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- Holloway, P. W. & Peluffo, R. O. (1964) Abstracts Sixth International Congress of Biochemistry, IUB Vol. 32, p.577, VII-68.Google Scholar